氯沙坦对高糖诱导NRK-52E细胞结缔组织生长因子及E钙黏蛋白表达作用研究.doc

氯沙坦对高糖诱导NRK-52E细胞结缔组织生长因子及E钙黏蛋白表达作用研究 广东潮安县华侨医院 陈根本 郭少川 广西医科大学第九附属医院内分泌科 梁华晟 钟宇华 苏会旋 【摘要】　目的　探讨氯沙坦对高糖诱导大鼠肾小管导管上皮细胞株NRK-52E结缔组织生长因子（CTGF）抑制作用。方法　作用72h后，Western blot检测空白组（0mmol/L葡萄糖）、正常葡萄糖组（5mmol/L葡萄糖）、高糖干预组（25nmol/L葡萄糖）、氯沙坦干预组（10nmol/L氯沙坦）、氯沙坦高糖干预组（10nmol/L氯沙坦预处理后在高糖培养）中CTGF及E钙黏蛋白在NRK-52E细胞表达。细胞ROS含量应用CM2H2DCFDA试剂盒检测。结果　高浓度葡萄糖上调CTGF表达，下调E钙黏蛋白表达， ROS含量显著上升。应用氯沙坦干预后可以下调高糖CTGF表达，上调E钙黏蛋白表达、细胞ROS含量下降。结论　氯沙坦可抑制高糖诱导NRK-52E细胞CTGF表达。 【关键词】　高糖；NRK-52E 细胞；结缔组织生长因子；氯沙坦 Effect of Losartan on high glucose induced the expression of connective tissue growth factor and E-cadherin in NRK-52E cHEN geng-ben, Liang Hua-sheng*, Zhong Yu-hua Su Hui-xuan Department of Endocrinology, the Ninth Affiliated Hospital, Guangxi Medical University, Beihai, Guangxi, 536000, China. 【Abstract】　Objective　To study whether losartan can protect NRK-52E from high glucose induced the expression of connective tissue growth factor(CTGF)and E-cadherin. Methods Western blot of CTGF and E-cadherin in control group(0 mmol/L glucose) , normal glucose group(5mmol/L glucose), high glucose group(25mmol/L glucose), losartan group (10nmmol/L losartan), losartan and high glucose group (10nmmol/L losartan and 25mmol/L glucose) had carried out . Then ROS were detected by CM2H2DCFDA after 72h. Result The expression of CTGF were up-regulated by hyperglycemia, and the expression of E-cadherin were down-regulated by high glucose. However losartan could down-regulate the expression of CTGF and up-regulate the expression of E-cadherin under high glucose condition. And losartan could decrease ROS under high glucose. Conclusion losartan maybe protect NRK-52E from high glucose which induced the expression of CTGF. 【Key words】　high glucose; NRK-52E;CTGF; losartan 作者姓名：陈根本；单位：广东潮安县华侨医院；科室：内科； 职称：内科副主任医师；通讯地址：广东潮安县华侨医院； 邮政编码515638；联系电话 E-mail：cgb3390463@ 结缔组织生长因子（connective tissue growth factor，CTGF）是即刻早期基因CCN家族的成员之一。CTGF与细胞外基质相互作用，可调节细胞间的粘附迁移、促进有丝分裂的发生、细胞活化和参与血管的生成。CTGF的表达水平与糖尿病多种组织纤维化的发生进展、血管生成及其预后密切关联[1-4]。E钙黏蛋白作