丙酮醛通过降低整合素β3的表达抑制内皮细胞的迁移.PDFVIP

J South Med Univ, 2015, 35(10): 1395-1399 doi 10.3969/j.issn. 1673-4254.2015.10.06 ·1395 · 基础研究 丙酮醛通过丙酮醛通过降低整合素降低整合素ββ33 的表达抑制内皮细胞的迁移的表达抑制内皮细胞的迁移 庞宁波，王立群，吴剑波 四川医科大学药物研究中心，四川 泸州 646000 摘要：目的探讨丙酮醛对内皮细胞迁移的影响。方法 浓度梯度的丙酮醛（0、25、50、100和200 μmol/L）刺激体外培养人脐静脉 内皮细胞（HUVECs）24 h ，划痕实验和Transwell 迁移实验检测丙酮醛对HUVECs 迁移的影响；免疫印迹检测整合素β3 的表达 变化；并采用β3 的抗体LM609 进行干预，观察LM609 对HUVECs 迁移的影响。结果 丙酮醛以浓度依赖的方式显著降低 HUVECs 的迁移（P0.05）；丙酮醛以浓度依赖和时间依赖的方式显著降低了整合素β3 的表达（P0.05）；β3 的特异性抗体 LM609显著抑制了HUVECs 的迁移（P0.05）。结论 丙酮醛通过降低整合素β3 的表达抑制了HUVECs 的迁移。 关键词：丙酮醛；人脐静脉内皮细胞；整合素β3 Methylglyoxal inhibits human umbilical vein cell migration in vitro by down-regulating integrin β3 PANG Ningbo, WANG Liqun, WU Jianbo Drug Discovery Research Center, Sichuan Medical University, Luzhou 646000, China Abstract: Objective To explore the effects of methylglyoxal on endothelia cell migration. Methods Human umbilical vein endothelial cells (HUVECs) were stimulated by serial concentrations of methylglyoxal (MGO, 0, 25, 50, 100 and 200 μmol/L) for 24 h, and the cell migration was assessed by scratch wound and Transwell assay. The expression of integrin β3 in the treated cells was examined by immunoblotting, and the effect of an anti-β3 antibody, LM609, on cell migration was investigated. Results Methylglyoxal significantly inhibited HUVEC migration in a concentration-dependent manner (P0.05). Methylglyoxal decreased the expression of integrin β3 in a time- and concentration-dependent manner (P0.05). LM609 also significantly inhibited HUVEC migration (P0.05). Conclusion Methylglyoxal inhibits HUVEC migration in vitro by down-regulating integrin β3 expression. Key words: methylglyoxal; human umbilical vein endothelial cells; integrin β3 ［1］ 1.1.1 细胞与试剂 HUVECs （Cascade Biologics）、 丙酮醛是一种葡萄糖代谢的中间产物 ，糖尿病时 丙酮醛在体内含量显著增高［2］。丙酮醛