SeMNPV组织蛋白酶基因的克隆表达及其对病毒杀虫的影响.PDFVIP

SeM NPV 组织蛋白酶基因的克隆表达及其对病毒杀虫的影响 2 2 1 1 张海元 , 黄柏青 , 梅春蕾 , 张忠信 ( 1. 中国科学院武汉病毒研究所 ,湖北武汉 43007 1 ;2 . 长江大学医学院 ,湖北荆州 434000) Cloning , Expression and Function Analysis of Cathepsin Gene Encoded by Spodoptera exigua Nucleopolyhedrovirus ZHAN G Haiyuan1 , 2 HU AN G Boqing2 , M EI Chunlei1 , ZHAN G Zhongxin1 ( 1. Wuhan I ns t i t ute of Vi rology , Chi nese A ca demy of S ciences , Wuhan , 43007 1 Chi na; 2 . M e d ical S chool , Yang tz e Uni vers ity , ) J i ngz hou , H ubei , 434000 , Chi na Abstract : A p ut ative vcat hep sin ( vcat h ) gene of S p od op te ra ex i g ua n ucleop oly he d rov i r us ( SeMN PV) wa s cloned , sequenced and exp re ssed . The re sult s showed t hat t he gene size wa s 10 14 nucleotide s , enco din g a 338 amino acids p ut ative p rot ein . The amino acid sequence align ment of t en baculoviral vCA T H s showed t hat t he p rimary st r uct ure an d t he cat alytic sit e s were con served . A p holygenetic t ree of t he se vCA T H s con st ruct ed by u sin g maximum p ar simony a naly si s indicat ed t hat t he vcat h gene of SeMN PV had a diff erent evolutionary hi story f ro m t hat of ot her N PV s. When lat e 3t h in st ar S p o d op te ra ex i g ua larvae were inf ect ed wit h SeMN PV μ co mbin ded wit h 1. 2 L exp re ssed bact eria an d medium , t he mort alit y wa s increa sed 9 . 2 1 % , an d L T50 wa s 12 h short er t han t he cont rol . The vCA T H exp re ssed in p ro karyot e ha s a significant eff ect on t he killing s