脂多糖干预气道上皮细胞细胞因子及蛋白激酶C表达的研究.docVIP

脂多糖干预气道上皮细胞细胞因子及蛋白激酶C表达的研究 王聪丽 邱小华 蔡颖,况九龙(330006 南昌，南昌大学第二附属医院呼吸内科) [摘要] 目的 探索脂多糖（Lippolysaccharide LPS）干预气道上皮细胞细胞因子分泌及蛋白激酶C(protein kinase C PKC)的表达。方法 培养A549细胞株，用不同浓度的LPS干预A549细胞2h,4h,8h,24h,30h,48h以后收集细胞上清液及细胞质总蛋白，用ELASA方法检测A549细胞IL-1、IL-6、IL-8、IL-11的分泌量，用western blot方法检测相应的PKC的表达量。结果 不同浓度LPS干预A549细胞相同时间后细胞因子IL-1，IL-6，IL-8，IL-11的分泌量及PKC的表达量之间的差异均有统计学差异（P＜0.001）；PKC蛋白的表达量和IL-11的分泌量之间具有相关性，相关性分析具有统计学意义（P＜0.05）。结论 LPS干预细胞因子IL-1，IL-6，IL-8，IL-11的分泌及PKC的表达，且LPS干预细胞因子IL-11的分泌可能和PKC信号通路有关。 [关键词]： A549细胞；蛋白激酶C；炎症因子；脂多糖 [中图法分类号]：R563.3 Expression of protein kinase C and cytokine in airway epithelial cell interfered by LPS Wang Congli Qiu Xiaohua Cai Ying Kuang Jiulong（The Second Affiliated Hospital To Nanchang University Nanchang 330006 China） [Abstract] Objectives: to explore expression of PKC and cytokine in airway epithelial cell interfered by LPS. Methods: A549 cell strain was cultivated and interfered by different concentrations LPS after 2h,4h,8h,24h,30h and 48h. We collected culture medium and total protein in the cell，detected volume of cytokines IL-1、IL-6、IL-8、IL-11 secreted by A549 cell using enzyme-linked immunosorbent assays (ELISA).Meanwhile, we measured expression of PKC by western blot. Results: There were significantly differences in volume of cytokines IL-1，IL-6，IL-8，IL-11 and expression of PKC interfered by various concentration of LPS in same period（all P values＜0.001）.Expression of PKC was correlated with volume of cytokine IL-1(P0.05).Conclusions: Secretion of cytokine IL-1，IL-6，IL-8，IL-11 and expression of PKC are interfered by LPS. Secretion of IL-1 interfered by PKC may be correlation with PKC signal pathway. [Key words] A549 cell；protein kinase C；cytokines；Lippolysaccharide； Supported by the General Program of ?National Natural Science Foundation of China；Corresponding Author：Cai Ying，Tel E-mail： HYPERLINK mailto:caiying5540@ caiying5540@ [基金项目]：国家自然科学基金项目：H0118） [通信作者]：蔡颖，邮箱 caiying5540@ 气道上皮细胞是气道壁的表层细胞和理化屏障，易受外界因素