富含S-层蛋白乳酸菌的鉴定及其消化酶耐受性研究.PDF

※生物工程 食品科学 2011, Vol. 32, No. 19 165 - 1 1 1,2 1,2,3, 肖 荣 ，范郁冰 ，王远亮 ，李宗军 * (1.食品科学与生物技术湖南省重点实验室，湖南农业大学食品科学与技术学院，湖南 长沙 410128 ； 2.湖南省发酵食品工程技术研究中心，湖南 长沙 410128 ； 3. 国家植物功能成分利用工程技术研究中心，湖南农业大学，湖南 长沙 410128) 通过平板划线分离法从新鲜牛奶中筛选获得一株乳酸菌菌株，命名为 M8 ，SDS分析该菌株富含 S- 层蛋白。结合表型特征、生理生化特征与 16S rRNA 基因序列分析法，鉴定该菌株为短乳杆菌(Lactobacillus brevis) 。对其人工消化液耐受性分析，结果显示：其对人工胃液(3.0g/L ，pH2.5) 具有良好耐受能力，能在人工 肠液(10g/L ，pH8.0) 中增殖，且S- 层蛋白具有耐消化酶酶解能力。提示 S- 层蛋白作为保护屏障，可抵抗消化酶的 作用 。 乳酸菌；S - 层蛋白；消化酶；耐受 Identification of a Lactic Acid Bacteria Strain Rich in Surface (S)-Layer Protein and Its Tolerance to Digestive Enzymes 1 1 1,2 1,2,3, XIAO Rong ，FAN Yu-bing ，WANG Yuan-liang ，LI Zong-jun * (1. Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China ；2. Hunan Provincial Engineering and Technology Center for Fermented Food, Changsha 410128, China ；3. National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, China) Abstract：A strain of lactic acid bacteria was isolated from fresh milk by plate streaking method. The isolated strain was named as M8, which was rich in S-layer protein according to sodium dodecyl sulfonate polyacrylamide gel electrophoresis (SDS- PAGE) analysis. The strain was identified as the species of Lactobac