苍耳子、田基黄中黄酮类物质提取含量测定和对比研究.PDFVIP

中国农学通报 2014,30(6):306-313 Chinese Agricultural Science Bulletin 苍耳子、田基黄中黄酮类物质提取含量测定及对比研究 张 成，朱科谕，张庞文，叶慧颖，史明敏，齐 晓，刘謦松 （北京师范大学珠海分校工程技术学院生物工程系，广东珠海519087） 摘 要：为考察苍耳子和田基黄中黄酮提取优化的研究和总含量的测定，及对两者黄酮总含量的对比研 4 究。通过芦丁作为标准物测定总黄酮含量，采用L (3 )正交试验法确定乙醇浸提回流法的最佳浸提工艺 9 条件。试验结果显示，苍耳子最佳浸提条件为乙醇浓度为60% ，提取温度70℃，提取时间4 h ，料液比为 1:30 (g/mL) ，此条件下苍耳子总黄酮提取率为3.89% 。田基黄最佳浸提条件为：乙醇浓度为50% ，提取 温度为80℃，提取时间为1 h ，料液比为1:50 (g/mL)。以总黄酮浸提率作为考察指标，苍耳子最佳浸提工 艺下总黄酮的提取率为3.89% ；而田基黄总黄酮提取率为4.31%。结果表明田基黄中黄酮含量较于苍耳 子高。本工艺简单可行，有较好的参考价值。 关键词：苍耳子；田基黄；黄酮；提取工艺；紫外分光光度法；正交试验 中图分类号：TS201.2 文献标志码：A 论文编号：2013-1487 The Study on Determination Content of Flavonoids Extracts from Fructus xanthii and Hypericum j aponicum thunb Zhang Cheng, Zhu Keyu, Zhang Pangwen, Ye Huiying, Shi Mingmin, Qi Xiao, Liu Qingsong (DepartmentofBioengineering,SchoolofEngineeringTechnology,BeijingNormalUniversity, Zhuhai Guangdong 519087) Abstract: The purpose of this paper was to investigate the best conditions of extracting the total flavonoids content for Frutus Xanthii and Hypericum j aponicum, and make a comparison about the total flavonoids in two 4 plants. Using the way of ethanol reflux condensation and applying rutin as the standard, L (3 ) was adopted to 9 confirm the best optimum extraction process condition extracting of active ingredients. The experimental results showed that the Xanthium optimum extraction conditions was the ethanol concen