芥菜型油菜雄性不育系与甘蓝远缘杂交胚培养及早代育性鉴定.PDF

园 艺 学 报 2010 ，37 （10）：1661 –1666 Acta Horticulturae Sinica 芥菜型油菜雄性不育系与甘蓝远缘杂交胚培养 及早代育性鉴定 王涛涛，蔡晓峰，张俊红，李汉霞，叶志彪* （华中农业大学园艺植物生物学教育部重点实验室，华中农业大学园艺林学学院，武汉430070 ） 摘 要：为了将优异的芥菜型油菜雄性不育性转育到甘蓝中，对甘蓝和芥菜型油菜不育系远缘杂交 胚的发育情况进行了研究，结果发现杂交胚发育 10 d 左右后开始败育。在杂交胚发生败育之前进行胚离 -1 -1 体培养，在 MS 基本培养基中添加 1 mg · L BA ，0.1 mg · L NAA 和活性炭的条件下，得到了远缘杂交 胚发育而成的植株。进而利用石蜡切片对远缘杂交植株的不育性进行了显微观察，发现其败育的时期发 生在雄蕊原基分化时期。 关键词：甘蓝；芥菜型油菜；雄性不育；胚培养 中图分类号：S 635 文献标识码：A 文章编号：0513-353X （2010 ）10-1661-06 The Culture and Early Male Sterile Identification of Distant Hybrid Embryos Derived from Brassica oleracea var. capitata L. and Male Sterile Line in B. juncea * WANG Tao-tao ，CAI Xiao-feng，ZHANG Jun-hong ，LI Han-xia ，and YE Zhi-biao （Key Laboratory of Horticultural Plant Biology，Ministry of Education ，Huazhong Agricultural University ，College of Horticulture and Forestry ，Huazhong Agricultural University ，Wuhan 430070 ，China ） Abstract ：The development of the distant hybrid embryos derived from Brassica oleracea var. capitata L. and male sterile line of B. juncea were investigated in this study. The results showed that the hybrid embryos began to abort in approximate 10 days after pollination. Some hybrid plantlets were obtained by -1 -1 using ovule rescue culture in vitro in MS media supplemented with 1 mg · L BA ，0.1 mg · L NAA and activated charcoal. The development of anther in distant hybrid plants were examined using paraffin section，and the abortion was found in the differentiation stage of stamen primordium. Key words ：Brassica juncea ；male sterile ；cabbage ；ovule rescue culture 十字花科作物存在明显的杂种优势，杂种一代的生产主要利用自交不亲