茶多酚双液相氧化制取茶色素工艺研究-南京农业大学学报.PDF

( ) 南京农业大学学报　2004 , 27 2 : 99～104 　 Journal of Nanjing Agricultural University 茶多酚双液相氧化制取茶色素工艺研究 李立祥 , 萧伟祥 (安徽农业大学农业部茶叶生物化学和生物技术重点开放实验室 , 安徽 合肥 230036) ( ) 摘要 : 研究了茶多酚双液相氧化制取茶色素工艺。结果表明 : 放大实验较优化实验茶色素中茶黄素 TFs 含量更高 ; 反应 液中 TFs 萃取效率以第 1 次最高 , 其他各次效率依次降低。对茶色素进行 TrisHCl (pH 80) 洗涤 , 其洗涤效率也以第 1 次 最高 , TFs 含量随洗涤次数增多呈现递增趋势 , 但被洗液体积呈递减趋势 , 并存在脱没食子酰基作用。 关键词 : 茶多酚 ; 儿茶素 ; 酸性氧化 ; 茶色素 ; 茶黄素 ; 双液相 ; 工艺 中图分类号: S5711012 　　　文献标识码 : A 　　　文章编号 : 1000 2030 (2004) 02 0099 06 Study on technology of preparation and extraction of tea pig ments from tea polyphenols oxidized in twoliquidphase system LI Lixiang , XIAO Weixiang ( Key Laboratory of Tea Biochemistry and Biotechnology , Ministry of Agriculture , Anhui Agricultural University , Hefei 230036 , China) Abstract : The technology of preparation and extraction of tea pigments from tea polyphenols oxidized in twoliquidphase system was studied. The results showed that there was higher content of theaflavins (TFs) in tea pigments from magnified test compared with optimized test. The extraction efficiency of TFs in tea pigments was the highest in first extracted reaction liquid , and the others were reduced in file. When tea pigments were washed with TrisHCl buffer (pH 80) , the washing efficiency was the highest in first washing and the contents of TFs in creased with washing times , but the volume of washed liquid was decreased accordingly , and there was degalloyl group action duri