顶空吸附吧法在沙枣花精油提取中的应用研究.docVIP

顶空吸附法在沙枣花精油提取中的应用 摘 要：目的 寻找沙枣花精油提取产率高，成本较低的方法，应用顶空吸附法对其精油的提取效果进行研究，为沙枣花的开发利用做一参考。方法 根据顶空吸附法原理，采用自制的实验装置，选用活性炭、大孔树脂作为吸附剂，石油醚、正己烷作为洗脱剂提取沙枣花精油，与水蒸气蒸馏法进行提取产率的对比，通过GC（Gas chromatography气相色谱法）鉴定精油主香成分—肉桂酸乙酯及其含量，确定最佳提取效果。结果 顶空吸附法提取的沙枣花精油主香成分是肉桂酸乙酯；大孔树脂作为吸附剂，正己烷作为洗脱剂时，精油提取产率为1.5%，肉桂酸乙酯的相对含量最高；活性炭作为吸附剂，石油醚作为洗脱剂时，300目活性炭产率为1.0%高于150目活性炭，其产率为0.8%，且300目活性炭作为吸附剂肉桂酸乙酯的相对含量高于150目活性炭；顶空吸附法提取效果优于水蒸汽蒸馏法，其产率为0.25%。结论 顶空吸附法可用于沙枣花精油提取，选大孔树脂作为吸附剂，精油提取产率优于活性炭，且此方法效果优于水蒸气蒸馏法，成本较超临界CO2萃取法低，性价比较高。 关键词：顶空吸附法；沙枣花；精油；提取率 The application about the head-space absorption method on extracting the essential oils of Elaeagnus angustifolia. blossom Abstract:Objective To study the method that the yield should be higher and the cost lower, the HAS(head-space absorption)was used to extract the essential oils of the Elaeagnusan gustifolia L. Method With the theory of the HAS being the basis,a device was designed by ourselves to extract the essential oils with the active carbon and the macroporous as adsorbent.Compared contents of the essential oils extracted by the SD(steam distillation)with the HAS, the GC(Gas chromatography)was used to identify the quality. Result With the method of the HAS,the main constituents of the essential oils being extracted was the ethyl phenylacrylate. The yield of it being extracted by the macroporous resin was 1.5%,150 mesh carbon as adsorbent 0.8%,the 300 mesh carbon as adsorbent1.0%,while the yield extracted by the SD (steam distillation)was 0.25%— the least of the all. Conclusion The method of the HAS could be used to extract essential oils of the Elaeagnus angustifolia L.The effect of new method with the macroporous resin and active carbon as adsorbent has an advantage over the SD and the supercritical CO2 extraction which cost more. Key words: Head-space absorption method; Elaeagnus angustifolia L.; the yield of the essential oils 沙枣(Elaeagnus angustifolia L.)是胡颓子科植物，沙枣树抗风沙、耐盐碱、耐干旱、易繁殖，适应性强而成为沙荒地、盐碱地造林的优良树种，在我国集中分布在西北荒漠半荒漠地区，是重要的防沙治沙植物。沙枣花银黄色，钟形，长5 mm左右，花期长约20 d左右，具有