基于cdte量子点的致病菌及巨噬细胞检测方法研究-营养与食品卫生学专业论文.docxVIP

摘要 摘要 性，简化了传统巨噬细胞的提纯步骤，在荧光倒置显 巨噬细胞，发展了一种用于监测机体免疫应激变化的 DNA杂交分析，磁性纳米颗粒，沙门氏菌，李斯特 像 Ⅱ Illll Illll l I II II I ll II IIII IIl Y1 889446 Since food··borne diseases caused by food··borne pathogen is one of the biggest dangers t0 food safety which has been concemed by all the countries，and each reported detection methods for food—borne pathogen has its shortcomings，developing a higher sensitivity,wider applicability,more rapid and convenient new detection method is very meaningful in order to meet the need of detection．The application of semiconductor quantum dots in life science has caused extensive concern at home and abroad，especially in the the application research as fluorescent probe，because of its good biocompatibility,strong light stability and hi曲yield． However，sensitivity of single quantum dot labeling technology in many application studies is not ideal，and there is little report about the quantum dot assembly technic which can significantly improve the detection sensitivity．Based on this，we used nano-probe technology and fluorescence immunoassay in DNA hybridization analysis，and made sequence—specific oligonucleotides of Salmonella，Staphylococcus aureus and Listeria monocytogenes弱the model analytes to establish new ultra-trace amount analysis system．Meanwhile，we used CdTe QDs as fluorescent marker to analyse the images of different physiological status of macrophages from mouse，and further broaden the application of quantum dots． Firstly,we synthesized CdTe QDs in an aqueous solution and characterized by TEM and fluorescence spectra．Then one nanogold Was connected witll hundreds of quantum dots to construct signal amplified probes using short DNA chain．Combining、)l，itll the Staphylococcus aureus specific sequences DNA capture probes，we captured specific sequencestarget DNA by hybridization to form a”sandwich¨composites Which were fixed on the micro well plate and detected the concentration of target DNA which was quantified by the fluorescent intensity o