免疫层析快速检测呋条喃唑酮代谢物的方法研究-生物化学与分子生物学专业毕业论文.docxVIP

AbstractImmunochromatographic Abstract Immunochromatographic test strip has a high specificity and sensitivity．This method enjoys the advantages of convenience，the large capacity of analysis and the detection of low cost．What’S more，its qualitative detection does not require auxiliary equipments，and the sample preparation step is simple and convenient．This ideal for field testing may not only provide serialized products and technology products can be commercialized，but also to help the detection for the industry to create economic value for society． We designed two immunochromatographic rapid test strips for AOZ with gold-magnetic composite particles and CdTe quantum dots instead of the colloidal gold-labeled monoclonal antibody AOZ，respectively．It combines llano—markers and immune chromatography，marked bY magnetic microspheres and CdTe QDs fluorescence—labeled antibody．Comparing with the same colloidal gold labeled，it improved the sensitivity．In qualitative analysis process，the former can be directly judged by the naked eye and the latter Can be tested through a portable ultraviolet light；In quantitative analysis process，the former can be numbered by magnetic analysis instrument，which Can be read by a fluorescence reader,respectively，on the chromatographic carrier number and the three-dimensional magnetic fluorescent signal，got the signal values directly，．We obtained the quantitative detection value through the establishment of a standard CHI The magnetic multilayer metal composite particles with uniform size and good dispersion were synthesized and successfully conjugated with the AOZ monoclonal antibody．As to explore the best coupling conditions，we got these as follows：pH 7．4 for the environment，l mg GoldMag can be the optimum content of the composite particle surface with the 1 70 lag AOZ-McAb conjugation，the contection content of antibody was 480 lag the coupling ratio was 35．43％．the blocking reaction needed 50 min in the Pbs(5％BSA，pH=7．4)and finall probes sav