内蒙古荒漠草原根际及非根际土壤微生物群落对不同放牧强度的呢响应-生态学专业毕业论文.docxVIP

AB AB STRACT This study is to research the influence different soil microbial community in rhizosphere and non—rhizosphere of Stipa Breviflora Desert Steppe with different grazing intensity,based on the continuous grazing for eight years on the sample field under human’S contr01．We choose the representative rhizosphere and non-rhizosphere soil from Desert Steppe plants to make deep comparison based on the different grazing intensity simulated on field．We used both traditional dilution-plate-method and Molecular Identification of species to compare the quantity,composition and diversity of soil microbe in rhizosphere and non-rhizosphere on different grazing conditions and different depth of the soil sample and to clarify the relation between grazing and changes Oll soil microbial community．The main conclusions are as follows： (1)The grazing intensity is now affecting the quantity of soil bacteria．In concrete，the quantity of soil bacteria increases under light grazing while the quantity under moderate and severe grazing reduces rapidly(even more obvious on topsoil samples)．In 0·1 0cm soil layer,quantity of soil bacteria under light grazing is higher than the soil bacteria under moderate and severe grazing by 38．19％．65．83％and 47．4％．The quantity of Bacillus Atrophaeus increases obviously in the rhizosphere soil of Stipa Breriflovathe under light grazing．It is the fundamental reason why the quantity of the non—rhizosphere soil bacteria increases under light grazing． (2)Grazing influence is more obvious on topsoil．Grazing reduces the quantity of soil fungi。In 0-1 0cm soil layer,the quantity reduces by 27．88％一42．92％because of grazing．This reduction is caused by the reduction of the quantity of Penicillium， Alternaria and Trichoderma in Stipa Breriflova rhizosphere soils． (3)Grazing decreases the quantity of actinomycetes only on the surface soil。In 0-10cm soil layer,the quantity of soil acfinomycetes is reduced by 25．61％，19．50％ and 32．93％under light grazing，moderate graz