ISSR标记鉴别砂梨种质资源的研究.PDFVIP

中国农学通报 2012,28(01):207-211 Chinese Agricultural Science Bulletin ISSR 标记鉴别砂梨种质资源的研究 1,2 1 2 吴 炼 ，袁德义 ，黄 笛 1 2 （中南林业科技大学林学院，长沙410004 ；湖南农业大学园艺园林学院，长沙410128 ） 摘 要：为有效区分和利用亲源关系较近的砂梨品种，利用简单重复序列(ISSR)进行多态性、遗传相似性 和聚类分析。从100 多条ISSR 引物对16份繁殖系材料DNA 模板进行扩增和电泳检测，结果表明，有16 条引物均能扩增出有较高稳定性、重复性和丰富多态性的条带，总数为135条，多态性达70.4% ，每条引 物扩增3~8 条条带；16 个砂梨品种间的遗传一致度的变化范围在0.6667~0.9630 ，遗传距离的变化范围 在0.0377~0.4055 ；‘华高’和‘新高’砂梨品种具有最高的遗传一致度(0.9630) ，它们之间的遗传距离也最 小(0.0377) ；‘华高’和‘雪青’砂梨品种的遗传距离最大为0.4044 ，相应的最低遗传一致度为0.6667。 关键词：砂梨；简单重复序列；遗传相似性；聚类分析；种质资源 中图分类号：S661.2 文献标志码：A 论文编号：2011-2594 Inter-simple Sequence Repeat Analysis on Pyrus pyrifolia Cultivars Germplasm 1,2 1 2 Wu Lian , Yuan Deyi , Huang Di 1 ( College of Forestry, Central South University of Forestry and Technology, Changsha 410004; 2 College of Horticulture, Hunan Agricultural University, Changsha 410128) Abstract: In order to efficiently distinguish and use cultivars germplasm of Pyrus pyrifolia with a near genetic relationship, the polymorphism and cluster analysis based on genetic similarity matrices of inter-simple sequence repeat (ISSR) markers were used on 16 cultivars. It showed that 16 pairs of ISSR primes produced 135 amplified fragments of which 70.4% were polymorphic. The average number of alleles per ISSR locus was range from 3 to 8. The genetic similarity coefficients among the 16 cultivars ranged from 0.6667 to