超声集成丙醇-硫酸铵双水相体系从苦荞麦苗.PDFVIP

110 2009, Vol. 30, No. 02 食品科学 ※工艺技术 高云涛 1,2 ，李干鹏1,2 ，李正全2 ，唐光阳1,2 ，张宝通1 (1.云南民族大学化学与生物技术学院，云南 昆明 650031 ；2.云南民族大学应用化学研究所，云南 昆明 650031) ：本实验利用超声提取集成丙醇- 硫酸铵双水相体系对苦荞麦苗中总黄酮进行提取分离，研究丙醇 - 硫酸铵 双水相的形成和提取条件对总黄酮得率的影响。结果表明，本方法可有效地从苦荞麦苗中提取总黄酮，提取率为 1.86%，提取物中总黄酮含量为 19.3%，明显高于回流提取法。提取物对脂质过氧化具有良好的抑制作用，最大 抑制率为 6 8 . 2 % ，方法操作简便、条件温和，环境友好。 ：超声波提取；双水相分离；苦荞麦苗；总黄酮；抗氧化 Study on Extraction of Total Flavonoids from Tartary Buckwheat Seedling Using Ultrasonic Coupled with Propyl Alcohol -Ammonium Sulfate Aqueous Two-phase Separation and Antioxidant Activity of Total Flavonoids Extract 1,2 1,2 1 1,2 1 GAO Yun-tao ，LI Gan-peng ，LI Zheng-quan ，TANG Guang-yang ，ZHANG Bao-tong (1.School of Chemistry and Biotechnology, Yunnan Nationalities University, Kunming 650031, China ； 2.Institute of Applied Chemistry, Yunnan Nationalities University, Kunming 650031, China) Abstract：The ultrasonic extraction technology coupling with propyl alcohol-ammonium sulfate aqueous aqueous two-phase separation for extracting total flavonoids from tartary buckwheat seedling was studied. The results showed that under the optimum conditions, the extraction rate of total flavonoids is 1.86 % and their content in extract is 19.3 %, which is much higher than that extracted by circumfluence of solvent. Moreover, the total flavonoids extract could singificant1y inhibit lipid peroxidation (LPO) induced by ·OH free radical generation system. In a word, this method is simply, mild conditions and environmentally friendly. Key words：ultrasonic extraction ；a