超高效液相色谱法同时测定饮料中5种人工合成色素.PDFVIP

※分析检测 食品科学 2011, Vol. 32, No. 04 177 5 张 婉，王 覃，杜 宁，周 悦，李津廷，张经华 * (北京市理化分析测试中心，北京 100089) 目的：建立超高效液相色谱同时测定饮料中5 种人工合成色素(柠檬黄、苋菜红、胭脂红、日落黄、亮 蓝) 的检测方法。方法：样品采用聚酰胺吸附法提取色素，通过Waters ACQUITY UPLCTM BEH C 18 反相色谱柱分 离，以甲醇 -0.02mol/L 乙酸铵缓冲溶液为流动相进行梯度洗脱，于254nm 和 629nm 波长处检测。结果：5 种人工 合成色素在 5.5min 内完成分离，3～80mg/L 范围内呈良好线性关系(r ＞0.9995) ，检出限为0.03～0.10mg/L ，平均 回收率为97.4% ～107.3%，RSD 为 0.22% ～3.20% 。结论：该方法简便、快速、分离效果好、灵敏度高，适用 于饮料中人工合成色素的测定。 超高效液相色谱法；人工合成色素；饮料 Determination of Five Synthetic Pigments in Beverage by Ultra Performance Liquid Chromatography ZHANG Wan ，WANG Tan ，DU Ning ，ZHOU Yue ，LI Jin-ting ，ZHANG Jing-hua* (Beijing Center for Physical and Chemical Analysis, Beijing 100089, China) Abstract ：Objective: To develop an ultra performance liquid chromatography (UPLC) method for the determination of five synthetic pigments such as tatrazine, amaranth, carmine, sunset yellow and brilliant blue in beverage. Methods: Synthetic pigments in beverage were extracted by polyamide adsorption method, separated on a Waters ACQUITY UPLCTM BEH C18 column by gradient elution using a mobile phase made up of methanol and 0.02 mol/L ammonium acetate, and detected at 254 nm and 629 nm. Results: The five synthetic pigments were separated in 5.5 min. The UPLC method exhibited excellent linearity over a range of 3 －80 mg/L (r ＞ 0.9995). The detection limit, recovery rate and relative standard deviation (RSD) of this method were 0.03 －0.10 mg/L, 97.4% －107.3%, and 0.22%－3.20%, respectively. Conclusion: The analytical method is simple, accurate, sensitive and suitable for the determination of pigments in beverage. Key words ：ultra performance liquid chromatography ；synthetic pigment ；beverage 中图分类号：O657. 63