互花米草sos1基因和hkt1基因的克隆及耐盐转基因水稻分析-海洋生物学专业论文.docxVIP

烟 烟 台 大 学 硕 士 学 位 论 文 i i Abstract The highly salt-tolerant halophytes Spartina alterniflora is as a material. According to its characteristis of a high degree of salt excretion and salt tolerance, the key salt genes SaSOS1, SaHKT1 related to ion transport with Na +、K +are cloned. A plant expression vector is built for the rice experiments by agrobacterium-mediated genetic transformation. As to Climate change and irrational use of land resources, the area of soil salinization continue to increase. We rsearch halophytes and its unique salt-related gene, to improve and screen crops with better salinity characteristics effectivly.That is an important strategy for effective use of saline. Futher more, it is expected to lay the theoretical foundation for salt exploitation and utilization of genetically modified crops. The results are as follows: The full length of the SOS1 gene and the HKT1 gene are cloned. A plant expression vector of p-super1300+ is constructed. A detailed analysis of the Agrobacterium-mediated genetic transformation are discussed in all aspects of the regeneration rate. The callus induction rate was 60%.The esistant callus rate is 62%.The regeneration rate is 6.2%. We got 470 regenerated plants by Agrobacterium-mediated experiment. 108 regenerated plants were selected randomly to PCR. The positive transgenic seedlings rate was 91%. Keywords： genetical modification, salt tolerance, rice ,SaSOS1,SaHKT1 目 录 摘 要 I HYPERLINK \l _bookmark0 ABSTRACT I HYPERLINK \l _bookmark1 文献综述 3 HYPERLINK \l _bookmark2 .植物盐害机理 3 HYPERLINK \l _bookmark3 1.1 渗透胁迫与渗透平衡的调节 3 HYPERLINK \l _bookmark4 1.2 离子胁迫与离子稳态的重建 4 HYPERLINK \l _bookmark5 1.3 氧化胁迫与活性氧在体内的清除机制 5 HYPERLINK \l _bookmark6 2.SOS 基因家族的结构特征与植物耐盐关系 6 HYPERLINK \l _bookmark7 2.1 SOS 基因家族的研究与发现 6 HYPERLINK \l _bookmark8 2.2 SOS1 基因的结构特征与植物耐盐关系 7 HYPERLINK \l _bookmark9 2.3 SOS2 基因的结构特征与植物耐盐关系 7 HYPERLINK \l _bookmark10 2.4 SOS3 基因的结构特征与植物耐盐关系 8 HYPERLINK \l _bookmark11 2.5 SOS4 基因的