HPLC同时测定黄檗种子中4种内源植物激素.pdfVIP

Hans Journal of Agricultural Sciences 农业科学, 2017, 7(3), 238-244 Published Online June 2017 in Hans. /journal/hjas /10.12677/hjas.2017.73032 Determination of Four Endogenous Phytohormones in Seeds of Phellodendron amurense by High Performance Liquid Chromatography (HPLC) Guixin Zhang, Fei Wang, Yuhong Zhang* Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin Heilongjiang th th th Received: Jun. 7 , 2017; accepted: Jun. 20 , 2017; published: Jun. 28 , 2017 Abstract Four endogenous phytohormones: Gibberellins, indoleacetic acid, zeatin and abscisic acid in the seeds of Phellodendron amurense, were examined by High Performance Liquid Chromatography (HPLC) to provide effective and reliable methods for identification of phytohormones in plant seeds. The four analytes were extracted from P. amurense seeds by 80% methanol and C-18 solid extraction column, and then separated by Agilent C18 column, using methanol, acetonitrile, water (1:1:8, V/V/V) as mobile phases. Detection wavelength: GA was 206 nm, IAA was 225 nm, ABA and Zeatin were 254 nm. First, the HPLC preseted 206 nm (GA) and 225 nm (IAA) as detection wave- lengths, and when the sample ran for 7 minutes, the detection wavelength was changed to 254 nm. This method had been applied to the determination of gibberellin, indole acetic acid, zeatin and abscisic acid in the seeds of P. amurense, and the recovery rate of 4 kinds of endogenous phyto- hormones was more than 93%, relative standard deviation less than 4.1%. The results showed that phytohormones was well separated, and the ideal quantitative data of phytohormones in P. amuren