毛冬青总皂苷对大鼠脑缺血再灌注损伤的保护作用及其机制研究.docx

毛冬青总皂苷对大鼠脑缺血再灌注损伤的保护作用及其机制研究 摘要：为研究毛冬青（Ilex pubescens Hook et Arn.）总皂苷对脑缺血再灌注损伤的保护作用，并初步探讨其保护作用机制，将试验大鼠随机分为假手术组、模型对照组、毛冬青总皂苷高、中、低剂量组及阳性药物对照组，运用大鼠大脑中动脉栓塞，制作脑缺血再灌注损伤模型，缺血10 min后，毛冬青总皂苷给药组及阳性药物组分别于舌下静脉给予高、中、低剂量的毛冬青总皂苷及阳性药物（香丹注射液），假手术组、模型组给予等体积的生理盐水。缺血60 min后再灌注，24 h后，每组取8只大鼠断头取脑，TTC染色，测定脑梗死体积;取血离心后，取血清，比色法测血清超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活性及丙二醛（MDA）的含量;每组取2只大鼠运用病理组织形态学检验，观察毛冬青总皂苷对脑缺血再灌注损伤大鼠脑组织细胞损伤的影响。结果表明，毛冬青总皂苷不同剂量组与模型对照组相比，其脑梗死面积减小，病理变化较轻;血清SOD的活性显著升高;毛冬青总皂苷高剂量组与模型对照组相比较，其MDA含量显著降低。说明毛冬青总皂苷对脑缺血再灌注损伤具有一定的保护作用，其保护机制与毛冬青总皂苷抗氧化作用及增强氧自由基的清除有关。 关键词：毛冬青（Ilex pubescens Hook et Arn.）总皂苷;脑缺血再灌注损伤;保护作用 中图分类号：R285.5 ? ? ? ?文献标识码：A ? ? ? ?文章编号：0439-8114（2014）21-5200-04 DOI：10.14088/j.cnki.issn0439-8114.2014.21.039 Protective Effects of Total Saponins from Ilex pubescens on Ischemia Reperfusion Injury of Rats and Its Mechanism LI Hong-lianga，HE Fang-xingb，CHENG Qi-laia （a.College of Pharmacy;b. Bascial medical college， Gannan medical College， Ganzhou 341000， Jiangxi， China） Abstract： To study the protective effect of total saponins from Ilex pubescens Hook et Arn on cerebral ischemia reperfusion injury and the potentially mechanism， the rats were randomly divided into sham operation group， model group， saponins of total saponins from Ilex pubescens Hook （high， middle， low） dose group and positive medicine control group. Same volume solvent or total saponins from Mao Dongqing was given in high， middle and low dosage， or positive medicine such as was given Xiangdan was injected sublingually 10min later of operation. The rats cerebral ischemia reperfusion model was made by middle cerebral artery embolization. ?After ischemia for 60 mins and reperfusion for 24 hours， 8 rats were taken from every group and removed brain by decapitation， then dyed by TTC and measured the volume of cerebral infarction， the activity of SOD， the content of GSH-Px and MDA in serum by colorimetry. 2 rats were taken from each group for histopathological examination. The effects of total saponins of Mao Dongq