羊毛防毡缩用蛋白酶的化学修饰-生物技术通报.PDF

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN 年第 期 2012 2 羊毛防毡缩用蛋白酶的化学修饰 陈忍忍 范雪荣 王强 袁久刚 朱怡然 （江南大学 生态纺织教育部重点实验室，无锡 214122） 摘 要 : 为减少防毡缩整理中蛋白酶对羊毛纤维主体结构的破坏作用，分别研究了戊二醛、微生物谷氨酰胺转氨酶（MTG ） 和水溶性碳二亚胺（EDC ）对蛋白酶Savinase 16L 的化学修饰，以期达到增大蛋白酶分子量，从而将水解作用限制在纤维表面的目 - 的。主要通过体积排阻色谱、SDS PAGE 谱图以及荧光光谱研究修饰酶的分子量和结构变化。结果表明，戊二醛不能对蛋白酶分 子进行有效修饰 ；MTG 会被蛋白酶水解，无法催化酶分子间发生共价交联 ；而碳二亚胺既可以使蛋白酶分子间发生交联，又能将 含有伯胺基的大分子修饰剂偶联到酶分子上。 关键词 : 蛋白酶 化学修饰 戊二醛 碳二亚胺 微生物谷氨酰胺转氨酶 羊毛 Chemical Modification of Protease Used for Shrink Resistance of Wool Chen Renren Fan Xuerong Wang Qiang Yuan Jiugang Zhu Yiran （ - ， ， ， 214122 ） The Key Laboratory of Science and Technology of Eco Textile Ministry of Education Jiangnan University Wuxi - Abstract: Glutaraldehyde, microbial transglutaminase （MTG） and water soluble carbodiimide （EDC） were employed to modify the - protease used in anti felting finishing of wool, with the purpose of increasing its molecular weight and restricting its action to the wool surface. The molecular weight distribution and structural changes of the modified protease were characterized by size exclusion chromatography, SDS- PAGE and fluorescence spectrum. The results showed that glutaraldehyde was not an efficient crosslinker for the protease, since no increase in the molecular weight was achieved. When MTG was used for the modification of the protease, it could