辐射与离体培养筛选水稻抗稻瘟病突变体SCREENING-OSTIgov.PDF

CNIC-01481 CSNAS-0135 辐射与离体培养筛选水稻抗稻瘟病突变体 SCREENING FOR RICE MUTANT OF RESISTANCE TO PIRICULARIA ORYZAE BY IRRADIATION AND IN VITRO TECHNIQUE (In Chinese) 中 国 核 情 报 中 心 China Nuclear Information Centre CNIC-01481 CSNAS-0135 辐射与离体培养筛选水稻 抗稻瘟病突变体 王彩莲 徐 刚 陈秋方 金 卫 （浙江省农业科学院原子能利用研究所，杭州，310021 ） 摘 要 不同类型和品种的水稻成熟胚培养，其愈伤组织和绿苗再生能 力有很大差异。水稻成熟胚诱导培养的最适培养基为LS-5 ，用 γ 射 线（100，150 Gy ）辐照，有促进绿苗分化和植株再生作用。花药 愈伤组织经30 Gy γ 射线处理可提高连续培养的绿苗分化率。水稻 继代培养的愈伤组织，经稻瘟病粗毒素处理，对游离氨基酸分析表 明，有 14 种氨基酸可定量分析，并以丝氨酸、谷氨酸的含量为最 高，分别占总量的20%左右。对照中，抗病品种氨基酸含量均高于 感病品种。粗毒素处理后，抗病品种氨基酸含量下降，而感病品种 的氨基酸含量却增加，品种珍龙 13 还出现对照中没有的精氨酸。 抗稻瘟病离体筛选，使R2 植株抗病能力有不同程度提高，并获得5 个抗病突变体。 1 Screening for Rice Mutant of Resistance to Piricularia oryzae by irradiation and in vitro technique (In Chinese) WANG Cailian XU Gang CHEN Qiufang JIN Wei (Institute for Application of Atomic Energy, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021) ABSTRACT The ability of callus formation and green plant regeneration was very different for different rice types and varieties in mature embryo. LS-5 was optimum medium for mature embryo. Increase in plant regeneration capacity was found with 100, 150 Gy gamma rays. The differentiation and regeneration of green plants were obviously improved when the anthers on induction medium were with 30 Gy of gamma rays. The change of free amino acids in subcultured callus tissue in rice were investigated after callus were treated with cutu