基于外切酶和纳米材料信号放大技术的核酸修饰酶活性检测新方法研究-分析化学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 广西师范大学硕士学位论文Studies 广西师范大学硕士学位论文 Studies on new methods for the detection of nucleic acid modifying enzymes activity based on the signal amplification technology of exonuclease and nanomaterials M．S．degree candidate：Yefei Ma Supervisor：Associate Professor LiangLiang Zhang Major：Analytical Chemistry Grade：20 1 3 Abstract Biosensor technology with the advantages of has short analysis time，cost-efficiency，good selectivity and high sensitivity，has greatly promoted the application of Analytical Chemistry in the field of life sciences．Due to the advantages of without isolation,simple operation，Can be performed in-situ measurement，low cost and SO on，fluorescence sensing technology has been widely applied for detection of nucleic acids，proteins，bioactive small molecules，viruses，etc． The introduced Nanomaterials with special optical and electrical characteristics provides a new perspective to construct the optical biosensor for Analytical Chemistry．In recent years，nucleic acid-mediated signal amplification method provides for the highly sensitive detection of biological important technical support，it has been widely used in various types of biomolecules detection．Gene modification process is closely related with the development of cancer，and genetic modification subject to various nucleic acid modifying enzymes coordinately regulated． Therefore，to establish new methods of nucleic acid modification techniques activity detection is important in the early diagnosis and treatment of cancer and other serious diseases，as well the pathogenesis of research． In this hesis，nanomaterials and nucleic acid signal amplification technology were employed for fluorescence sensing system．This thesis has developed threenew methods for detecting Dam methyitransferase，base excision repair enzyme activity analysis and Inhibitor screening．The main contents ofthis work can be concluded as follows： Part one：In this work，a simple fluorescence strategy based on the graphene oxide(GO)