基于几种双功能硼酸衍生物的酶固定和安培酶电极研究-分析化学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 2．基于对巯基苯硼酸 2．基于对巯基苯硼酸(MBA)与蛋白酶糖基的亲和反应及其巯基 与纳米金(Aun锄。)的强键合作用，一步合成了GOx—MBA．Aun锄。复合 物，部分滴干于MWCNTs和电镀金层(Aupl砒e)修饰的金电极上，以 CS层加固后得到CS／GOx—MBA—Aun加o／AUplate／MWCNTs／Au电极。在 第一代生物传感模式下，对葡萄糖的响应灵敏度为121．5吣mM-1 cm～，LDR为10．0 gM～3．1 mM，LOD为1．0 gM(酣Ⅳ=3)。 3．基于聚呋喃．3一硼酸(PFBA)的糖基亲和及氧化聚合性质，一步 合成了尿酸氧化酶(UOx)一PFBA—PdNPs生物纳米复合物。所制备的 CS／UOx．PFBA—PdNPs／Pdplate／MWCNTs／Au酶电极对尿酸的安培响应 灵敏度为490 laA mM-1 cm-2，LDR为1．0 laM～2．5 mM，LOD为O．1 gM(S／N=3)。将该酶电极和Pt／MWCNTS／Au电极组装了尿酸／氧气生 物燃料电池，在模拟生理条件下其开路电位为0．394 V，短路电流密 度为857 p,A cm～，最大功率密度为70雎W cm～。 关键词：安培酶电极；生物燃料电池；生物纳米复合物；生物传感； 硼酸衍生物；钯；金；葡萄糖；尿酸 II 万方数据 ABSTRACTBoronic ABSTRACT Boronic acid derivatives can react with 1，2一or 1，3-diols，which has found wide applications in separation chemistry,synthesis chemistry and analytical chemistry．The amperometric enzyme sensors use enzymes as their molecular recognition element and output amperometric signals from redox chemistry of the enzyme systems，which possess many advantages such as high sensitivity and selectivity． Enzyme immobilization is the key step in fabricating an amperometric enzyme sensor,and enzyme immobilization through various nanocomposites has become the recent research foCUS in this field．In this thesis，first we briefly review the recent progress of electrochemical enzyme sensors and boric acid—diols reactions，then some researches are conducted on enzyme immobilization and biosensing mainly based on the reactions of several bifunctional bronic acid derivatives with the glycosyl groups of enzymes． The contents are as follows． 1．A glucose oxidase(GOx)一poly(3一anilineboronic acid)(PABA)一Pd nanoparticles(PdNPs)bionanocomposite was synthesized in an one。step manner based on the chemical oxidation polymerization of 3-anilineboronic acid(ABA)by Na2PdCl4 and the chemical interaction of glycoprotein enzyme’S diols with boronic acid groups．An appropriate amount of the bionanocomposite was cast·-coated Oil a Pd—-plated III 万方数据 万方数据 万方数据 multiwalled multiwalled carbon nanotubes(MWCNTs)一m