基于生物网络的大鼠再生肝细胞基因表达谱数据分析-生物学·动物学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 ABSTRACTThe ABSTRACT The liver of mammalian has an impressive ability to regenerate．Rat liver Can restore to the mass and functionality of organ within 7 days after partial hepatectomy,called liver regeneration．The abnormal of the liver function would result in a variety of liver diseases．The prognosis of hepatopaths which were treated with hepatectomy or living donor liver transplantation，were closely related with the regenerative capacity of liver．Therefore， uncovering molecular mechanism of liver regeneration was one of the research hotspots in biology and regenerative medicine fields at present． Many types of liver cells participate in liver regeneration．One of the most important cells is hepatocyte．As a terminally differentiated cell，most of the hepatocytes were in resting state at physiological condition．After partial hepatectomy,the residual hepatocytes of human and rodent would immediately start mitosis and cell proliferation．Liver Can restore to the original mass and function in a relatively short period．Although liver regeneration has been an interesting research topic over a century,the regulation mechanisms of the initiate，proliferation， differentiaion and termination of hepatocytes during LR remain to be elucidated． In this thesis，we gain gene expression data of regenerating hapatocyte．Then，we use biological network analysis methods to cluster the gene expression profile、ⅣitIl time series feature，analyze gene CO—expression network and protein-protein interaction network of rat hepatocyte during liver regeneration．This study provides theoretical basis for mining regulation mechanisms of liver regeneration． The contributions of thiS thesis are as follows： (1)In this study,the classic 2／3 partial hepatectomy model in rats is used．Hepatocyte is isolated at different time points during liver regeneration．Then gene expression changes were measured at Oh，2h，6h，12h，24h，30h，36h，72h，120h，168h after partial hepatectomy and sham operation wi