层流状态下高低切应力对动脉重构及血管细胞粘.pdf

2011;31(8) 南方医科大学学报（J South Med Univ ） ·1349 · ·基础研究· 层流状态下高、低切应力对动脉重构及血管细胞粘附分子-1表 达的影响 刘 俭，刘 莹，宾建国，李美瑜，黄瑞珠，伍巍兰，袁 野，宾建平 南方医科大学南方医院心内科，广东 广州 510515 摘要：目的制备小鼠腹主动脉狭窄模型，探讨近似层流状态下高、低切应力对动脉重构及VCAM- 1表达的影响。方法20 只小鼠随机平均分为狭窄1 d 组、7 d 组、14 d 组和假手术对照组，用动脉银夹建立腹主动脉局部狭窄模型，超声检测狭窄 近心端和狭窄处血流动力学参数，计算切应力值；血管标本行HE 染色和内皮血管细胞粘附分子-1（VCAM- 1）免疫组织化 学染色，定量分析动脉病理结构的改变及内皮VCAM- 1表达的强度。结果狭窄动脉近心端和狭窄处分别形成低、高切应 力血流区，与对照组比较，随着作用时间的增加，狭窄近心端动脉内中膜逐渐增厚、内皮VCAM- 1表达逐渐增强，而狭窄处 动脉无明显结构改变及内皮VCAM- 1表达。结论血流动力学改变，尤其是低切应力可较早引起动脉重构，可能通过内皮 VCAM- 1的介导作用导致动脉粥样硬化的发生、发展，而高切应力则可能有抗动脉粥样硬化作用。 关键词：低切应力；高切应力；血管重构；内皮血管细胞粘附分子-1；动脉粥样硬化 中图分类号：R392 文献标志码：A 文章编号：1673-4254（2011）08-1349-04 Effects of high and low shear stress on vascular remodeling and endothelial vascular cell adhesion molecular-1 expression in mouse abdominal aorta LIU Jian, LIU Ying, BIN Jian-guo, LI Mei-yu, HUANG Rui-zhu, WU Wei-lan, YUAN Ye, BIN Jian-ping Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To establish a mouse model of abdominal aorta stenosis and analyze the alterations in the arterial wall response to high and low shear stress. Methods Twenty mouse were randomized equally into 4 groups, including 3 test groups (1, 7 and 14 day groups) with surgically induced stenosis of the abdominal aorta, and a sham-operated group without stenosis. The hemodynamics and the internal diameter of the blood vessel were measured by color Doppler flow imaging. The wall shear stress was calculated by Poiseiulle hydrodynamics formula (τ =η × 4 × V /D). Pathological examination and immunohistochemistry were performed to observe the arterial m