应用CRS-RFLP技术检测hOGG1c326位点的单-中国工业医学杂志.PDF

　　　2004 10 17 5 　　Chinese J Ind Med　Oct 2004, Vol.17 No.5 ·293 · CRS-RFLP hOGG1c.326 张忠彬, 朱　人, 夏昭林 (, 　200032) 　　:　 (CRS-RFLP) 。　CRS-RFLP , hOGG1c.326 , 。　CRS-RFLP 。　CRS-RFLP , 。 :;; :R394 　　:A　　:1002-221X (2004)05-0293-04 Genotype identifying of hOGG1c .326 with method of CRS-RFLP ZHANG Zhong-bin, ZHU Ren, XIA Zhao-lin (Department of Occupational Health, School of Public Health, Fudan University, Shan hai 200032, China) Abstract:Objective To e plore strategy for identifying single nucleotide polymorphism with technique of introducing mismatched bases to create restriction site combined with restriction fragment length polymorphism (CRS-RFLP)and its applying.Method　With method of CRS-RFLP, the genotype of hOGG1c.326 was identified, and the results were compared with these of RFLP not introducing mismatched bases in primer.Resu t 　The results were easily recognized and consistent with these of not introducing mismatched bases in the primer.Conc usion　It suggested that this method is good for identifying found single nucleotide polymorphism e tensively e ist- ing in sequence of genes. Key words:Restriction fragment length polymorphism;Created restriction site;Single nucleotide polymorphism 　　 (created , restriction site-RFLP, CRS-RFLP) 、, 。CRS-RFLP RFLP , , , hOGG1 [1] 。 c.326 , CRS-RFLP , (environmental 。 genomic project, EGP), 1　 1.1 　 (single nucleotide polymor- 、、 phisms, SNPs)。 152 , 57 , 95 (restriction fragment length polym