NGS云端分析软体讲习.PDF

NGS 雲端分析軟體講習 李彥樑(Jack) 威健生技 Welgene Biotech. Welgene Biotech. Co. Ltd. .tw Next Generation Sequencing (NGS) NGS (massively parallel sequencing) 8 一次讀10條序列 , 每條= 75bp －75-100 bp/read －500 bp/read －100,000,000 reads/run －1,000,000 reads/run －10-25 Gb/day Illumina Hiseq AB SOLiD Roche 454 DNA/RNA Sequencing Reads/Tags 100G Bases per run per machine 5 G Bases ~ 2 Gb zipped sequence file Re-sequencing 原始序列 計算區域內的短序列數目 GATCATGTCACTATACG 分析mismatch位置與數量 8 (10條 reads, 對應到Ref Genome 上 每條~100bp) (Mapping) Reference DNA/RNA Sequence Reads/Tags Accessible to everyone: easy, cost-effective Address NGS data challenges •Millions of sequence need to be processed Accelerate data analysis for customers •Web-based (no software) •Cloud infrastructure (no hardware) Next-gen sequence analysis and visualization History 2009 Founded + initial investment April 2010 Product launch Oct 2011 Invested by Oct 2011 Host the DNAnexus SRA Profile Headquarters Mountain View, CA Cloud Computing Service 的 雲端雲端運算說明： • 虛擬化技術快速部署資源或服 務 •動態的、可伸縮的擴充功能 • 按需求提供資源、按使用量付費 •使用者可以方便地參與 • 形態靈活，聚散自如