反式脂肪酸对内皮细胞脂肪酸组成的影响-食品科学.PDF

※营养卫生 食品科学 2013, Vol.34, No.19 275 反式脂肪酸对内皮细胞脂肪酸组成的影响 于化泓，梁忠爽，李湘梅，李 静，刘小如，刘 蓉，范亚苇，邓泽元* (南昌大学食品科学与技术国家重点实验室，江西 南昌 330047) 摘  要：采用气相色谱分析法(GC)检测不同反式脂肪酸(TFA)对人脐静脉内皮细胞(HUVEC)脂肪酸组成的影响。结 果表明：反油酸和反亚油酸作用于内皮细胞后，细胞中TFA 的含量大幅度增加，降低饱和脂肪酸和多不饱和脂肪酸 的含量，尤其是对二十碳五烯酸(EPA) 、二十二碳六烯酸(DHA) 、花生四烯酸(AA)影响明显。且两种反式脂肪酸 对细胞脂肪酸组成的影响有一定差异，反亚油酸作用后细胞中的多不饱和脂肪酸和单不饱和脂肪酸的含量要比反油 酸作用后高。 关键词：反油酸；反亚油酸；人脐静脉内皮细胞；脂肪酸组成 Effect of Trans-fatty Acids on Fatty Acid Composition of Human Umbilical Vein Endothelial Cells * YU Hua-hong，LIANG Zhong-shuang，LI Xiang-mei ，LI Jing，LIU Xiao-ru ，LIU Rong，FAN Ya-wei ，DENG Ze-yuan (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China) Abstract ：In this study, the effect of two different trans-fatty acids (TFA) on fatty acid composition of human umbilical vein endothelial cells (HUVEC) was determined by GC. The results showed that after HUVECs were treated by elaidic acid and linolelaidic acid, the content of trans-fatty acids rapidly increased and the content of saturated fatty acids and polyunsaturated fatty acids exhibited a significant reduction, especially the contents of EPA, DHA and AA were obviously affected. Both TFAs had different influences on fatty acid composition in HUVECs. Meanwhile, subj ected to treatment with linolelaidic acid, the contents of polyunsaturated fatty acids and mono-unsaturated fatty acids in HUVECs were much higher when compared with the treatment by elaidic acids. Key words ：elaidic acid ；linolelaidic acid ；human umbilical vein endothelial cells；fatty acid composition 中图分类号：R 151.2 文献标志码：A 文章编号：1002-6630(20 13)19-0275-04 doi:10.7506/spkx 1002-6630-20 13190