日本晴9311水稻重组自交系重要农艺性状qtl定位研究-生态学专业论文.pdf.docxVIP

ABSTRACTRice(Oryza ABSTRACT Rice(Oryza sativa L．)is one of the most important crops，and it is the staple food for more than half of the people worldwide．During the domestication of rice，two subspecies indica and japonica formed under natural and artificial selection．Crosses between indica rice and japonica rice often lead to inter-species hybrid sterility．Because rice is a monocot with 1 2 pairs of chromosomes(-430 Mb)，it has been widely used as model plant in research and breeding．Construction of linkage maps， mapping and cloning of genes or quantitative trait loci(QTLs)related to important agronomic traits are meaningful to the breeding of fice varieties 、析m good traits． To estimate the influences of segregation distorted markers on the building of linkage map and QTL identification，we designed a GoldenGate SNP array harboring 3 84 evenly distributed SNPs along chromosomes that selected and experimentally validated based on the SNPs between 93 1 1(indica)andNipponbare(japonica)to genotype the 298 stably inherited recombination inbred lines(RILs)derived from 93 1 1 (paternal)and Nipponbare(maternal)，of which，259 are from F5 generation，and 39 are from F6 generation．Moreover,we have also collected 1 4 agronomic traits in total for 1 52 randomly selected RILs， including one-thousand-grain weight(TGW)，the ratio of grain length to grain width(L／W)，panicle length(PL)，arista length(AL)，arista color (AC)，grain color(GC)，plant height(P均，flag leaf length(FLL)，flag 万方数据 leaf leaf width(FLW)，the ratio of the length of the flag leaf to its width (FLL／FLW)，tiller number(耵D，growth period(GP)，seed setting rate (sR)，and lodging rate fLR)．Aider bioinformatic analysis，323 SNPs in total were found to be polymorphic among RILs．Ultimately,294 SNPs were selected to build the linkage map with distance of 1,583．2 cM，of which，78．57％SNPs ale segregation distortedmarkers．The linkage map constructed here is highly consistent with the map built without segregation distorted markers in previou