酿酒酵母中β-胡萝卜素生物合成研究-制药工程专业论文.docxVIP

优秀毕业论文 精品参考文献资料 北京化工大学硕士学位论文导时间。通过菌体生长情况及产量的比较，确定使用 北京化工大学硕士学位论文 导时间。通过菌体生长情况及产量的比较，确定使用SD选择培养基， 发酵至12 h诱导后产量最高；在探究的最优条件下发酵菌株InvScl— GAB与InvScl—AEB(2A)，二者产量分别为2．9 mg·g。和2．8 mg·g～。 关键词：B一胡萝卜素，酿酒酵母，甲羟戊酸途径，酮康唑，FMDV 2A 序列 万方数据 ABSTRACTTHE ABSTRACT THE BIOSYNTHESIS OF[；-CAROTENE IN Saccharomyces ABSTRACT As a model organism for food safety，Saccharomyces cerevisiae has become a natural product biosynthesis preferred host cells．[3-carotene has drawn a lot of attention because of its high—efficiency oxidation resistance and high value—added features．With the synthetic biology，we used different sources of synthetic p-carotene gene to improve the titer of production． Specific work is as follows： After strain InvSc l—GAB begins to ferment 1 2 h，1 8 h，24 h，we added ergosterol inhibitors ketoconazole to give a final concentration of 3 0 mg·L一． Adding after 1 8 hours of fermentation，the yield of[3-carotene from experimental group was 20％higher than that from control group．The highest yield was 20 mg·L～． Connected all the genes from the MVA pathway by FMDV 2A sequence to express in the same reading frame．Constructed strain InvSc 1—2A—YBIE， InvSc l-2A—BYIE，InvSc 1-2A-YBIG and InvSc l一2A·BYIG in order to III 万方数据 北京化工大学硕士学位论文express 北京化工大学硕士学位论文 express the genes from Erwinia and the replacement of geranyl pyrophosphate synthase from different source． Constructed strain InvSc 1-YBIE(2A)，InvSc l—YBIE(2A)／pR-t-2A—i， InvSc 1·IE—YB(2A)，InvSc l—ABG(2A)，InvSc 1一AEB(2A、with optimized FMDV 2A sequence．The expression ability was improved by optimization of connection order and the involvement of genes from upstream．Besides，the expression ability of crtE was confirmed better by comparison of crtE from Erwinia and ggps from Blakeslea trispora． In this study,the best medium and the optimal indution time were also explored during the fermentation process of strain InvSc l—ABG(2A)．After comparing the cell growth and yield，the SD medium was performed better than YPD medium．Strain InvSc l—