葛根素对急性缺血再灌流损伤肾脏的保护作用.docVIP

葛根素对急性缺血再灌流损伤肾脏的保护作用 The protective effect of guggenin on the renal protection of acute ischemia 2011-09-15 jiang denglin is in her time Puerarin (universal law) from traditional Chinese medicine (TCM) in wild radix puerariae flavonoids extract, separation and purification of an injection, a large number of animal experiments and clinical research: puerarin can improve cardiac, cerebral ischemic diseases and its effect on the microcirculation [1] ; However, the active effects of the lipid peroxidation process and Na + -k + -atpase were not reported. This experiment to establish acute ischemia in the rat kidney perfusion model, observation of puerarin on acute ischemic renal perfusion SD rats again form function and renal tissue malondialdehyde (MDA) and the influence of Na + -k + ATPase activity, in order to investigate puerarin on acute ischemia and perfusion damage renal function protection and mechanism. One, material and method 1. Experimental animal: a total of 18 male SD rats, weight (180 ~ 200 g), choose 8 weeks, provided by experimental animal center of fujian medical university, were randomly divided into control group and the control group and treatment group. 2. Model: rats by pentobarbital sodium (40 mg/kg) free bilateral kidneys after intraperitoneal injection of anesthesia, silver clip block right renal arteries and veins let go after 30 minutes, observe the right kidney hyperemia, ligation of left renal arteries dnd veins, excision of the left kidney. Surgical group: dissociated bilateral kidney, expose 30 minutes to be suture, do not be treated? Treatment group: renal ischemia before tail intravenous injection puerarin (sino-foreign joint venture of yantai zhongee pharmaceutical co., LTD15 mg/kg), ischemia 30 minutes after intravenous additional same dose drugs, according to 30 mg/kg dose after intraperitoneal injection once every six hours? Control group: using the same method of injection for injection of saline, three groups of rats were executed