中药现代化技术.docx

收稿日期：2018-00-00;定用日期：2018-00-00;DOI：10.13550/j.jxhg基金项目：陕西省教育厅重点实验室项目（14JS024）；陕西省社会发展科技攻关项目（2016SF-347）；陕西省教育厅专项资助项目（14JK1194）；陕西中医药大学校内基金（14XJZR11） [作者简介] 赵鹏（1977- ），男，山西临猗人，教授，博士。研究方向为天然药用活性成分分离纯化。E-mail： HYPERLINK mailto:zhaopeng65@ zhaopeng65@联系电话 中药现代化技术 混合磷酸盐法制备款冬花磷酸酯多糖 201805300391 赵鹏，张丽华，王国全，张婷婷 （陕西中医药大学药学院，陕西咸阳 712046） 摘要:以款冬花多糖为原料，采用混合磷酸盐法制备了款冬花磷酸酯多糖。以磷酸基的取代度（DS）为指标，在单因素实验的基础上，采用响应面法对款冬花多糖磷酸酯化的合成工艺进行了优化。结果表明，最优合成工艺条件为：反应时间6.0h，反应温度86℃，尿素用量为反应物总质量的14.25%，m〔混合磷酸盐（磷酸二氢钠与磷酸氢二钠）〕:m（款冬花多糖）=1.5:1，m（磷酸二氢钠）:m（磷酸氢二钠）=2.0:1。在此条件下制备的款冬花多糖磷酸酯，DS平均值为0.697。抗氧化性实验结果表明：款冬花多糖磷酸酯对DPPH·最大清除率为98.91%，O2-·最大清除率为48.85%，·OH最大清除率为50.34%，均高于款冬花多糖。 关键词:款冬花；磷酸酯多糖；混合磷酸盐法；合成工艺；抗氧化性；中药现代化技术中图分类号：TQ28 文献标识码：A文章编号：1003-5214 (2018) 00-0000-00 Synthesis of Phosphorylated Polysaccharides from Tussilagofarfara by Mix-phosphate Method Zhao Peng, Zhang Li-hua, Wang Guo-quan, Zhang Ting-ting (School of Pharmacy, Shaan Xi University of Chinese Medicine, Xian Yang 712046) Abstract:Phosphorylated polysaccharides from Tussilagofarfara was synthesizedby mix-phosphate method.The synthetic process conditions was optimized via the response surface method. The research results show that 6.0h, 86℃, 14.25% of urea2.0 of mass ratio of mix-phosphate, 1.5 of mass ratio of mix-phosphate and polysaccharides were the optimum conditions. The DS of phosphorylated polysaccharides from Tussilagofarfara was 0.697. The antioxidant ability of phosphorylated polysaccharides from Tussilagofarfara had been improved.Its maximum removal rate for DPPH·,O2-·and ·OHwas 98.91 %,48.85 %,50.34 %. Key words: Tussilagofarfara; phosphorylatedpolysaccharides; mix-phosphate method; synthesis process; antioxidant activity; modernization technology of traditional Chinese medicines Foundation items:Educational Commission of Shaanxi (14JS024); Social Development by Department of Science and Technology of Shaanxi (2016SF-347);Shaanxi Provincial Department of Education Funding Project(14JK1194); Foundationof?Shaanx