糖原合酶激酶3β以cyclin D1依赖性方式引发人肺腺癌细胞株A549细胞周期阻滞系列.pdfVIP

204 Acta Physiologica Sinica , April 25, 2007, 59 (2): 204-209 Research Paper Glycogen synthase kinase 3β induces cell cycle arrest in a cyclin D1- dependent manner in human lung adenocarcinoma cell line A549 LI Jian-Sha, ZHU Min, TIAN Dan, WANG Man-Xiang, WANG Fang, LI Na-Ping, WU Ren-Liang* Department of Pathology, Tongji Medical College, Huazhong University of Science and Technology, and Key Laboratory of Pulmonary Disease of Ministry of Health of China, Wuhan 430030, China Abstract: The effect of glycogen synthase kinase 3β (GSK3 β) has been repeatedly implicated in cell proliferation, but studies on the effect of GSK3β in different cell lines with different stimuli have drawn different conclusions. To investigate the direct effect of GSK3β on cell growth in human lung adenocarcinoma cell line A549, we changed its activity by transient transfection with two kinds of GSK3β mutant plasmids, constitutively active form S9A-GSK3 β and dominant negative form KM-GSK3 β . Twenty-four hours later, cell counting, flow cytometry and Western blot detection were made respectively. The results showed that enhancing GSK3 β activity caused a decrease in cell number, as well as a higher percentage of cells at G1 phase. Further, the expression of cyclin D1 was down- regulated by GSK3 β. Taken together, our observations suggest that GSK3β may induce G1 cell cycle arrest in a cyclin D1-dependent fashion and therefore possibly plays a growth-inhibitory role in A549 cells. Key words: glycogen synthase kinase 3β; cell proliferation; cell cycle; cyclin D1 糖原合酶激酶 3 β 以细胞周期蛋白 D1 依赖性方式引发人肺腺癌细胞 A549 细胞周期阻滞 李建莎，朱敏，田丹，王满香，王芳，李娜萍，吴人亮* 华中科技大学同济医学院病理学系，卫生部呼吸系统疾病重点实验室，武汉430030 摘 要：对糖原合酶激酶3β (glycogen synthase kinase 3 β, GSK3β)在细胞增殖中的作用研究，在不同细胞系和不同刺激因素作 用下得出了不同结论，本文旨在探讨GSK3β 在人肺腺癌细胞系A549 细胞生长中的直接作用。A549 细胞瞬时转染持