黄孢原毛平革菌对大豆秸秆木质素降解的FTIR表征-食品科学.PDF

※生物工程 食品科学 2011, Vol. 32, No. 19 137 FTIR 王丽婷，徐 伟 * ，张玉强 (哈尔滨商业大学食品工程学院，黑龙江 哈尔滨 150076) 研究黄孢原毛平革菌(Phanerochaete chrysosporium Burdsall)对大豆秸秆中木质纤维素的降解条件。结果显 6 示：5g 秸秆粉中添加合成培养液 12mL、接种量为0.8mL(孢子悬液浓度 8.6 ×10 CFU/mL) 、培养基初始pH4.5 ，发 酵 10d 后，木质素降解率为 45.96% 。并采用傅里叶红外光谱分析(Fourier transform infrared spectroscopy ，FTIR) 检测降解后秸秆中官能团的变化，发现与木质素相关的谱峰(1099cm-1 -1 、1057～1038cm )相对强度减小，与苯环相 -1 -1 关的谱峰(1643～1608cm 、1510～1508cm )相对强度增加，表明部分大分子木质素裂解成小分子木质素或木质素单 体，对比其他谱峰相对强度的变化，发现木质素中苯环等环状化合物含量减少。 大豆秸秆；黄孢原毛平革菌；木质素；FTIR FTIR Characterization of Lignin Degradation Products from Soybean Straw Fermented by Phanerochaete chrysosporium Burdsall WANG Li-ting ，XU Wei* ，ZHANG Yu-qiang (College of Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China) Abstract：The degradation conditions of soybean straw lignin by Phanerochaete chrysosporium fermentation were studied in this paper. The results showed that addition of 5 g of soybean straw power to 12 mL of synthetic medium for fermentation at an initial pH of 4.5 and an inoculum size of 0.8 mL (8.6 × 106 CFU/mL spore suspension concentration) for 10 d proved optimal and the resultant degradation rate of lignin was 45.96%. Also, Fourier transform infrared spectroscopy (FTIR) was employed to changes in functional groups arising from lignin degradation. The results showed that the relative intensity of spe