氮素对初花后棉花叶片衰老的生理生化特征及蛋白质组效应研究作物产品安全生产调控工程专业论文.docxVIP

对15 对15 d、26 d、36 d叶龄的上部果枝叶衰老调控蛋白质的差异表达情况进行分析，共鉴 定426种差异表达蛋白，超过73％的蛋白覆盖度在10％以上。对这些差异蛋白质进行GO 聚类分析和Pathway分析，获得了较多的与棉花果枝叶片衰老相关的蛋白质，主要分布在叶 绿体、过氧化物酶体中，参与的生物过程为光合作用过程，其分子功能主要参与抗氧化酶活 性、过氧化物酶活性和氧化还原酶活性的调控。叶片衰老相关蛋白主要参与光合作用、氮代 谢、光合碳固定和过氧化物酶体等代谢通路。 4． 氮素对花铃期棉花主茎功能叶差异蛋白质组分析 采用iTRAQ技术对正常氮素和缺氮条件下，花铃期棉花主茎功能叶片进行差异蛋白质 组学分析，共鉴定出164个差异表达蛋白，其中上调70个，下调94个。结合蛋白质GO分 析中差异蛋白的细胞位置及功能分析，并经过Pathway分析，差异蛋白被富集到4条Pathway 通路，分别是核糖体、光合作用、细胞周期和嘧啶代谢。 关键词：棉花；氮素；衰老；叶片；差异蛋白质组学 万方数据 The The Physiological and Biochemical Characterization and Leaf Proteomein after Initial Flowering Regulated by Nitrogen in Upland Cotton Author：Liu Liantao Major：Crop Products Safety and Regulation Supervisor：．Professor Li Cun-dong Abstract CoRon is an important economic crop in China,premature aging caused a serious decline in the yield and quality,which affected the enthusiasm of cotton planter．Clear mechanism of coRon premature senescence，exploring effective ways of delaying aging，which has the important practical significance．眦s study Was conducted at the experimental farm of Agricultural University of Hebei(38．8080N，1 1 5．4090E)from 2011-2015．Using transgenic insect-resistant(Bt)coRon‘SCRC 28’as test material，4 nitrogen fertilizers(NO，N1，N2 and N3 with 0，1 20，240 and 480 kg．hm-2 nitrogen, respectively)were set up to establish different senescence groups．We examined the physiological，biochemical and proteomic changes in individual cotton locules and cotton bolls during the aging process after initial flowering．We studied plant morphology,ni仃ogen utilization,physiological and biochemical parameters，as well as used iTRAQ proteomics．In order to explain the cotton leaf senescence process and nitrogen regulation related by proteomics research．To provide theoretical basis for cotton molecular breeding and cotton cultivation technology based on molecular diagnosis．Our results are as follows： 1．Effect ofnitrogen on the physiology,nitrogen utilization and yield ofcoRon bolls Different senescence groups were organized under fie