不同水稻品种dehy基因的转化研究及后代分析植物学专业论文.docxVIP

万方数据 万方数据 河北科技大学硕士学位论文 II AbstractAbstract Abstract Abstract Rice is one of the three staple crops，which hold the world’S major land acreage， especially in China．With the development of transgenic technology,reconstruction of the rice genome aroused widespread attention，and GM technology is widely used in various aspects of fice production，to cultivate yield rice with features of drought tolerance，insect resistance，and herbicide resistance．Currently，rice has gradually become a model of transgenic research organism．However，rice has a wide range of different varieties，in process of tissue culture，different varieties showed quite different characteristics and plant regeneration ability；in genetic engineering process，different varieties conversion efficiency is also significantly different The experiment intends to use 6 different rice varieties-Kunhan 1，Handao 2， Hannuo 2，Hannuo 3，Suijing 8 and Jijing 88，and lead DEHY gene into their callus via Agrobacterium method to take tissue culture，in order to study difference in callus characteristics，tissue culture and regeneration capacity and conversion efficiency of rice varieties with different genotype．Meanwhile，use Tx，T2 generation of transgenic rice plants to take PCR identification，herbicide identification and southern blot analysis to determine the copy number and select a single copy of the plants homozygous，in order to provide more useful references for the applications of transgenic technology in rice breeding． The results showed that：(1)different rice varieties callus induction rates were quite different，the callus induction rate of Hannuo 3 was highest，amounting to 92．8％，while the callus induced rate of Kunhan 1 was relatively low，only 8 1．6％；(2)the terms of the phenotype and proliferation of different varieties of rice callus significantly different， Suijing 8 and Jijing 88 proliferative capacity were better；(3)different rice varieties have a greater ability to differentiate differences，Suijing 8 and Jijing 88