分散液液微萃取高效液相色谱法检测复杂基质中的几种小分子化合物药物分析学专业论文.docxVIP

以达到26倍和24倍。方法的线性范围为1～1000 以达到26倍和24倍。方法的线性范围为1～1000 pg／L，格列吡嗪和 格列齐特的检出限分别为1 pg／L和2 pg／L。加标回收率介于 91．8％～107．6％之间，日间RSD小于9％。该方法有效消除复杂基质 的干扰，简便快速。 关键词：分散液液微萃取；高效液相色谱；尿样；保健食品 ⅡI 万方数据 ABSTRACTDispersive ABSTRACT Dispersive liquid liquid microextraction(DLLME)is a new microextraction and environment friendly pretreatment method，which reported by Rezaee in 2006．It is simple，convenient，saves time and easy to operate，has excellent precision and good selectivity and owns multi—element enrichment capability and low detection limits．DLLME has a good compatibility characteristic to combine with high phase liquid chromatography(HPLC)，high phase liquid chromatography—mass spectrometry (HPLC—MS)， gas chromatography (GC)， gas chromatography—mass spectrometry(GC—MS)and SO on．It is applied widely，including environmental pollutants control，analytical chemistry， biochemistry，clinical medicine，pharmacological researches．In this paper， a method on dispersive liquid—liquid microextraction for separation and analysis of several small molecule compounds in complex matrix prior to determination by high performance liquid chromatography has been studied．It mainly consists of the following four parts： 1．The summary of several sample pretreatment methods，especially liquid phase microextraction(LPME)and dispersed liquid liquid phase microextraction(DLLME)． 2．A simple and fast carrier-mediated dispersive liquid-liquid microextraction method followed by high performance liquid chromatography(HPLC)was developed to selectively extract and measure urinary monoamine neurotransmitter metabolites(MNMs)．We IV 万方数据 optimized optimized factors influencing extraction such aS extraction and dispersant solvent type and volume，sample solution pH，carrier concentration， extraction time．We used tributyl phosphate(TBP)as a carrier and n-butanol and methanol as extraction and dispersive solvents．respectively． Using optimal conditions，enrichment factors for 5-hydroxyindoleacetic acid(5-HIAA)，vanilly