大鼠颈动脉球囊损伤后血管平滑肌细胞增生性衰老的实验研究心血管内科专业论文.docxVIP

山西医科大学硕士学位论文The 山西医科大学硕士学位论文 The study of vascular smooth muscle cell’S replicative senescence after balloon inj ury of rat carotid arteries Abstract Objective By the study of vascular smooth muscle cell’S replicative senescence after balloon mjury of rat carotid arteries，to approach the role of replicative senescence in vascular restenosis， and provide a new theoretical basis of vascular restenosis． Methods 40 male wistar rats al e randomly divided into two groups，one group 20 male wistar rats．The model of vascular restenosis established by balloon injury of rat carotid arteries are established．One group is experimental group(use neointima formation inhibitor)，the other group is control group(don’t use neointima formation inhibitor)．Every group is divided into 5 subgroup，measure the PCNA-positive and SA-13一Gal—positive in 2d l w 2w 4w 6w after the ballooninjury． Result The positive rate of PCNA reach the max in the two weeks，then with the passage of time gradually decreased；when we use neointima formation inhibitor(All—Trans Retinoic Acid)， Compared winl the control group PCNA-positive rate has dropped．In the early days of balloon injury,the SA-13-Gal-positive rate is low,谢nl the passage of time，SA-p—Gal positive rate gradually increased，Application of all-trans retinoic acid，compared谢t11 the experimental group， the SA-D-Gal positive rate Was tend to decrease． Conclusion Vascular smooth muscle eell proliferation and replicative senescence are both existed after balloon injury of rat carotid arteries．In the different periods of injury,they play different roles on the formation of restenosis．Vascular smooth muscle cell proliferation play a major role in the early time，and there are mainly replicative senescences in the later time．When we use all—trans retinoic acid，neointima formation is inhibited，and the phenomenon of replicative senescences is tend to decrease．朋1e phenomenon of replicative senescences maybe an important reason to oppose the restenosis． Keyword v