中国水仙ntsamt基因的克隆及转化金鱼藤的研究风景园林园林植物与应用专业论文.docxVIP

CON，rENTSCONTENTS CON，rENTS CONTENTS Abstract in Chinese I Abstract in English ．III 1 Introduce ． ．1 1．1 Aims and significance of reseaech ”1 1．2 Research progress at home and abroad ．1 1．2．1 Fragrance biosynthesis gene research progress key enzymes and genes ··l 1．2．2 The research progress of plant flowers SABATH methyltransferase ．2 1．2．3 Research progress of SAMT gene ··3 1．2．4 Research progress of Narcissus tazetta var．Chinensis Roem ．5 1．2．5 Research progress ofAsarina procumbens 一5 1．3 The technical route and the main content 6 2 Materials and Methods ．．7 2．1 Experiental materials 7 2．1．1 Plant materials 7 2．1．2 Strain and vector 7 2．1．3 Test reagents 7 2．1．4 Medium 8 2．2 Experimental methods ·8 2．2．1 The full—length cloning of the gene ． ·8 2．2．2 Plant expression vector cotruction of genes ．1 3 2．2．3 Genetic transformation mediated by Agrobacterium＆plant regenration ．．14 2．2．4 Molecular biology detection of resistant plant 1 6 3 Results and Analysis ． ． 一1 8 3．1 Extracting the total RNA 1 8 3．2 Cloning the full-lengtht of NtSAMT by RT-PCR method ．．1 8 3．3 NtSAMT gene sequence and its coding protein homology analysis 1 9 3．3．1 The full-length cloning of the NtSAMT gene 一1 9 3．3．2 NtSAMT gene homology analysis ． ． ．19 3．3．3 NtSAMT evolutionary tree encoding amino acid sequence analysis ．1 9 3．4 Plant expression vector construction of genes ．．20 3．5 Genetic transformation mediated by Agrobacterium＆plant regenration ．24 3．5．1 Ascertainment of Km selection pressure 24 3．5．2 Ascertainment of antibiotics 。27 3．6 Exogenous gene into the Asarina procumbens ．28 3．7 Resistance of the plant PCR detection 29 3．8 RT．PCR indentification of transgene plants ．．27 4 Discussion 。29 4．1 Study of floral gene separation method ．29 Ill 万方数据 东北农业大学农学硕士学位论文4．I．I 东北农业大学农学硕士学位论文 4．I．I Functional cloning ．29 4．I．2 Homology-based cloning 一29 4．1．3 Phenotypic differences cloning ．29 4．2 Research about floral genetic improvement 29 5 Conclution ．3 1 Acknowledgement 32 References ．33