tfcp211促进小鼠胚胎干细胞自我更新的相互作用蛋白的筛选与鉴定生物工程专业论文.docxVIP

中文摘要的表达可以影响Tfcp211促进mESCs自我更新能力。而免疫共沉淀的结果显示 中文摘要 的表达可以影响Tfcp211促进mESCs自我更新能力。而免疫共沉淀的结果显示 Tfcp211和MTAl在mESCs中有相互作用。 关键词：Tfcp211，46C mESCs，自我更新，MTAl 万方数据 Tfcp211促进小鼠胚胎千细胞自我更新的相互作用蛋白的筛选与鉴定Abstract Tfcp211促进小鼠胚胎千细胞自我更新的相互作用蛋白的筛选与鉴定 Abstract Embryonic stem cells(Embryonic Stem Cells，ESCs)can be carried out in vitro unlimited self-renewal，and meanwhile Embryonic stem cells ESCs call maintain the ability to differentiate into various cell types of the organism，and therefore embryonic stem cells ESCs in the field of regenerative medicine has broad application prospects．Preliminary experiments we have found that transcription factor Tfcp21 1 is key downstream target genes of the LIF／STAT3 signaling pathway,changing Tfcp211 expression levels can greatly affect the LIF／STAT3 signaling pathway to promote self-renewal of mouse ESCs function，but the role of Tfcp21 1 specific molecular mechanisms have not been reported at home and abroad．Whether there is a critical and new transcription factor for mESCs(mouse embryonic stem cell) self-renewal．Up to now,it has been disclosed in numerous downstream target genes of STAT3，only down Tfep211 expression，that it Call weaken the LIF／STAT3 signaling promotes mESCs self-renewal function，thereby inducing cell differentiation，And SO far identified genes that can induce mESCs differentiation are not many．Based the important role of ESCs in the self update of Tfcp211，combined with a variety of efticient gene editing tools，such as lentivirus expression system and PiggyBac Transposon systems，it is possible to discover new genes that are essential for maintaining the undifferentiated state of mESCs．This paper intends to use gene expression and interference technology to screen and identify the Tfcp211 to promote the interaction of self-renewal ESCs gene in mice，expect to find maintain the undifferentiated state of mouse ESCs new regulatory factors mechanisms．This paper selected MTAl，MTA2，MTA3 as target genes to study the mechanism of their inter