不同林分类型与取食刺激诱导下油松抗性相关酶系的动态变化生态学专业论文.docxVIP

Studies Studies on the DynaIIlic Changes About the Resistence Related Enzymes ofPinus tabulaeformis in Different Forest Types and Feeding Stimulation Conditions Author：．Zhang Bin Supervisor：Prof．Gao Baojia Major：Ecology Abstract This dissertation took Pinus tabulaeformis Carr．as research object in pure stand of Pinus tabulaeformis and Pinus tabulaeformis-Prunus sibirica mixed forest stand in Pingquan County,Hebei Province,selected 20 health pines in each stand and conducted on 4 treatments of leaf-cutting(30 pine caterpillars daily consumption simulation)，1 0 and 30 pine caterpillars(Dendrolimus tabulaeformis)feeding(selected branch feeding inside cages)with no treatment as contrl，each treatment repeated 5 times．Tbok 2a needle samples for examination and test on each pine of two stands in first ten days of June，July and August 201 5 respectively．In order to explore the performance and mechanism of resistance on Pinus tabulaeformis induced by feeding and leaf-cutting stimulation under nasal environmental and the impacts of forest stand types on induced resistance，the resistance related enzymes activity were measured by UV spectrophotometry and ELISA．Main results for the study are as follows： (1)In pure stand ofPinus tabulaeformis，POD，PPO were significantly expressed in needles which treated by leaf-cutting，and GST expressed obviously after the same treatment；POD，CAT and PPO were significantly expressed under 10 pine caterpillars feeding treatment，SOD was not only noticeably increased in feeding time but also after it；to stimulated by 30 pine caterpillars，CAT had get high level expression during feeding period in the needles，SOD，PAL and LOX expression were significantly increased in feeding or after feeding time． (2)In mixed forest stand，POD，CAT and SOD were prominently expressed both in leaf-cutting period and after treatment stage，PPO and LOX were obviously expressed only in feeding time；under the deal with 1 0 pine caterpillars，CAT and SOD were significantly expre