不同条件下猪精液质量及精子黏附蛋白基因家族表达的差异兽医专业论文.docxVIP

值)无显著差异。各组处理方法如下：①对照组：不作任何处理，采用原 值)无显著差异。各组处理方法如下：①对照组：不作任何处理，采用原 有饲养管理模式进行饲养及利用；②试验组一：正式试验期每天上午喂料 后1 h开始播放发情母猪叫声，持续30 min；③试验组二：正式试验期每天 拌料口服枸橼酸克罗米酚200 mg(每日所投喂药物量根据药物使用说明规 定和试验种公猪平均体重约为230 kg计算得到)，平均分为2次喂完；④ 试验组三：正式试验期喂料后1 h开始播放发情母猪叫声，持续30 min，同 时每天拌料口服枸橼酸克罗米酚200 mg(平均分为2次喂完)。结果表明， 单独播放发情母猪叫声可以使种公猪性欲增强，射出精子总数增多，但对 种公猪的其它精液质量指标，除了使第二周的VAP和第六周的VSL显著高 于对照组外(氏O．05)外，均不能产生显著的影响(尸O．05)。单独口服枸 橼酸克罗米酚时，第二周的VAP、第六周的VSL和畸形率显著高于对照组 (PO．05)，其它与对照组无明显差异(PO．05)。发情母猪叫声和枸橼酸克 罗米酚同时处理时，第二周的VAP和第六周的VSL显著高于对照组 (尸O．05)，其它与对照组无明显差异(胗O．05)。 关键词：猪精液品质精子黏附蛋白基因家族 万方数据 The The differences of Spermadhesins expression and quality of boar semen under different conditions ABSTRACT The routine quality indexes of semen have some limitations for the evaluation of boar fertility，SO it is very necessary to further improve the senlen quality analysis．The objective of this study was to detect the di疏rences of boar semen quality and Spermadhesins expression under different conditions．and to analyze the causes of the fluctuation in boar semen quality and reproductive performance，to provide scientific basis for the marker assisted selection of boar semen quality and fertility．Specific research contents include two parts： In the first part，the differences of quality and Spermadhesins mRNA expression level of fresh semen from Luchuan，Landrace and Duroc boar were evaluated．．Luchuan，Landrace and Duroc boar semen was coilected to test sperm motility,viability,deformity rate，and mRNA expreSSion of spermadhesins(semi。quantitative RT-PCR)．The significance of di虢rence was analyzed by the one-way ANOVA．The results showed that sperm defonllity of Luchuan boar was significantly lower than Landrace and Duroc俨O．O 1)：the AQN 1 mRNA expression level of Luchuan boar semen was significantiv 10wer than Duroc(PO．05)，the AQN3，AWN and PSP．II gene mRNA expression levels of Landrace boar semen were significantly higher than Duroc(AQN3、 则烈，氏O．05；PSP—II，火0．01)．In short，under the present exp嘶mental conditions，semen quality o