nrf2信号通路在葡萄籽原花青素降低铅诱导的大鼠肾脏氧化应激中的作用兽医学临床兽医学专业论文.docxVIP

CONTENTSCoNTENTS CONTENTS CoNTENTS Abstract in Chinese ．I Abstract in English ．． ．．． ．．． ． ”III 1 Introduction ． ．．．． ．1 1．1 Pb ．1 1．1．1 Pb and oxidative stress ． ． 1 1．1．2 Pb and apoptosis 1 1．1．3 The renal toxicity ofPb ．． 。2 1．1．4 Pb poisoningtreatmentprogress 2 1．2 GSPE ．． ．．．．．．．．．．．． 3 1．2．1 The antioxidant effect ofGSPE ．．．．．．．．．．．．．．．． ． ．．．．． 3 1．2．2 The else effect of GSPE ．3 1．3 Nrf2 signaling pathway ··4 1．3．1 The composition，function and activation ofNrf2 signaling pathway 一4 1．3．2 The effect ofNrf2 signaling pathway ． ．5 1．4 Objective and significance 6 2 Materials and Methods ． 7 2．1 Material ．7 2．1．1 Experimental animal ． ．．7 2．1．2 Experimental equipment 7 2．1．3 Primary reagent ·7 2．1．4 Pharmaceutical preparation ．．9 2．2 Methods ．．10 2．2．1 Experimental model ． ．10 2．2．2 Samples collection ． ． ．．． ．． 10 2．2．3 Determination ofrenal function ． ． 10 2．2．4 Determination ofoganism index ．． 。10 2．2．5 Determination ofPb content in kidney ．10 2．2．6 Tissues for histopathology ． 1 1 2．2．7 Determination of SOD，MDA，GSH，and GST levels in tisssues ．．1 1 2．2．8 Determination of apoptosis by TUNEL ． ．．．． ·． ”1 1 2．2．9 Determination ofNrf2 by immunohistochemistry ．． 12 2．2．10 Western blot 12 2．3 Statistics and analysis．． ． 15 3 Results．．． ．．．． ． ．．．．．．．．．．．．．．．．．．．．． 。16 3．1 The changes ofrat kidney damage “16 3．1．1 The changes oforgan index ．．16 III 万方数据 3．1．2 3．1．2 The changes ofrenal function “16 3．1．3 The changes ofPb content -17 3．1．4 The changes ofkidney histology ·18 3．2 The changes of antioxidant enzymes in kidney ·1 8 3．3 The change ofapoptosis ． ·l， 3．4 Effects of GSPE activation Nrf2 signaling pathway on renal injury induced by Pb ·22 3．4．1 The changes ofNrf2 in kidney ·zz 3．4．2 The changes ofriO．1，NQOI，and Keapl in kidney ”23 4 Discussion ．-25 4．1 Effect ofGSPE renal injury induced by Pb ·-25 4．2 E任Iect of GSPE antioxidant level in kidney ”25 4．3 Effect of GSPE on apoptosis induced by Pb ”20 4．4 Effect of GSPE Nrf2 signaling pathway ·z／ 5 Con