阪崎克罗诺杆菌表面蛋白的鉴定水产品加工及贮藏工程专业论文.docxVIP

ABSTRACTC幻，zD6口c纪r ABSTRACT C幻，zD6口c纪r J口施翻舫f is a new eme画ng opponlmistic food_bom pamogen widely existed in Powdered milk(dai啪products．It caIl cause many diseaSes in all age groups． A1mou曲this pathogen is not Very dallgerous to adults，the neonate and premature are at hi曲risk wrhen infected with this bact嘶um，which caIl cause life-tllreatening inf-e曲ons， such as s印ticemia，necrotizing enteroc01itis，and meningitis．In recent years，infection rate of C s以勋磁兢fhas greatly improVed，aIld has attracted the attention of relevant d印矧【ments of many countries in the wodd． The surface proteins are a class of pmteins those are bound t0 me ceU surface aRer synthesis． They are widespread in the cell wan surface of Gr锄-positive bacteria， Gra】m-negatiVe bacteria and archaea，having importallt向nctions in tems of the nutrient abso叩tion，basal metabolism and invadiIlg host．Due to the stimulation of the host immune system，exploration of Vaccine potentials of surface proteins of pathogens has become a hot research topic．Furcher，the surface protein has good immunogenicity，which will be an e日’ectiVe candidate ta唱et of detection．In C J口l沈以舫f，except 0mpA，little is known about the identity and如nction of other surface proteins． Tb s01Ve the problems mentioned aboVe，surface proteins of C．sakazakii were identi6ed using immunoproteomic tecllIliques．T11is work layS foundation for mrther inVestigations on the traceabinty，pathogenic mechanisms，control aJld preVention， the establishment of the detection memods of C s口施绷舫t In this study，5 food-bome isolates of C s口庇眈口衙f were selected．These矗Ve is01ates were：fixed wim formaldehydeand injected into New Zealand white rabbits to pr印are monoclonal antibodies．During immunization， the titers of the serum were monitored by indirect ELISA．Membrane proteins of fiVe isolates were extracted and desalted using commercial kits． One-dimensional electrophoresis (1-DE) were used to detenlline the quality of extracted proteins． Two—dimensional electrophoresis(2-DE)in combin