α横纹肌肌节肌动蛋白α平滑肌肌动蛋白和结蛋白在小鼠胚胎及生后小鼠心脏的表达组胚专业论文.docxVIP

山西医科大学 山西医科大学 硕士学位论文 Expression of a-sarcomeric actin，co-smooth muscle actin and intermediate filament protein Desmin in the prenatal and postnatal mouse heart Abstract Background：To inquire further into the functional and morphological establishment and maturation of the heart during the development，we investigated the spatiotemporal expression pattern of cc-sarcomeric actin(oc-SCA)．Ⅸ-smooth muscle actin(cc-SMA)and Desmin du ring the development of the mouse heart to find out the relationship of these protein expression with the specific functional properties and the development of the prenatal and postnatal mouse heart· Methods：Serial sections of prenatal and postnatal mouse hearts were stained with monoc]onal antibodies against o【-SCA。cc-SMA and Desmin． Results：ceSCA and oc-SMA expressed in the heart of ED9，the expression of cc-SCA is stronger in the ventricle，but with the extension towards the arterial pole of the outflow tract，the staining intensity gradually decreases．The expression of e-SCA in the atrium is 山西医科大学 山西医科大学 硕士学位论文 restricted to the dorsal and ventral walls，leaving the lateral walls negative．tn the sinus few 0[‘SCA positive cells with ry low staining intensity found．The expression pattern of a。SMA is similar to that of(x-SCA，except that its expression in the outflow tract is stronger than that of a—SCA and the sinus shows negative staining．With the development，the expression of c￡-SCA and(z-SMA gradually increases to reach its highest level at EDl2．Relatively higher expression intensity of a·SCA remains to the postnatal stages·In contrast，a．SMA expression gradually disappears after EDl 2 beginning in the interventricular septum and followed by the compact myocardium of the left ventricle and then，the right ventricle·Expression the trabecullar myocardium persists 10nger．Fragmented expression ofⅨ．SMA in the myofibrils still be detected month after birth-In the hea rI Of ED 9，Desmin expre