nadph氧化酶p47phox亚基在鼠衣原体生殖道感染和致病中的作用研究微生物学专业论文.docxVIP

AbstractChlamydia Abstract Chlamydia trachomatis is all obligate intracellular parasite and a major cause of human blin(hless and sexual仃ansmitted disease．C抛cJilD7竹口f西infection Can lead to endometritis，cervicitis and salpingitis in women，resulting in complications such as ectopic pregnancy and tubal factor infertility．The mechanisms by which C trachomatis organisms induce inflammatory pathologies in genital tract remain unknown．And no effective vaccine has been licensed until now．There is less genital tract inflammation in mice inoculated wim C trachomatis．And murine biovar C muridarum Can cause inflammatory pathologies that are similar to those in humans．Therefore C muridarum genital tract infection in mice has been used extensively as a model for investigating the mechanisms of C trachomatis pathogenesis and immunity． In this research，we investigated the role of NADPH(nicotinamide adenine dinucleotide phosphate)一oxidase in the process of C muridarum infection and pathogenesis．NCF 1(neutrophil cytosolic factor 1)-encoded p47phox(phagocyte oxidase)is an essential subunit of NADPH oxidase．Deficiency of p47phox Can severely compromise the function of NADPH oxidase．To study the role of NADPH oxidase in infection and pathogenesis of C muridarum in mouse， p47phox deficient mice and wild type mice were inoculated intravaginally with C muridarum．Vaginal swabs were taken on different days after infection to monitor C muridarum infection in the lower genital tracts．Mice were sacrificed in the late stage of infection and the mouse urogenital tract tissue were isolated for in situ gross examination of uterine hom dilation and hydrosalpinx．Urogenital tract tissue sections were made for microscopic evaluating of pathology and counting of different types of inflammatory cells．To study whether NADPH oxidase plays a role in prevention of chlamydial ascending infection,p47phox deficient mice and wild type mice were sacrificed in early stage of infection．Genital tract tissue of m 万方数