低温诱发肉鸡腹水综合征患鸡钙信号转导及心肌重塑的机制研究基础兽医学专业论文.docxVIP

境低温可在一定程度上促进肉鸡右心室心肌细胞和成纤维细胞增殖。维拉帕米可抑制心肌细胞和 境低温可在一定程度上促进肉鸡右心室心肌细胞和成纤维细胞增殖。维拉帕米可抑制心肌细胞和 成纤维细胞过度增殖，有效防止了右心肥大的发生。 6．本研究通过免疫组化和原位杂交技术初步探讨c-fos和c-jun在AS肉鸡右心肥大和衰竭 过程中的变化及维拉帕米对其表达的影响。结果发现：在22、29、36、43和50日龄时，与对照 组肉鸡相比，低温组肉鸡右心室心肌细胞c—fos／c-jun蛋白和mRNA的平均光密度值显著或极显著 升高(PO．05；P0．01)，其mRNA的阳性面积百分比也极显著升高(P．0．01)；与低温组肉鸡 相比，维拉帕米组肉鸡右心室心肌细胞c-fos／c-jun蛋白和mRNA的平均光密度值显著或极显著降 低(P．0．05；P0．01)，其mRNA的阳性面积百分比也极显著降低(P0．01)。 结果表明c．fos 和c-iun癌基因在右心肥大和衰竭中起着重要作用。维拉帕米可有效抑制c．fos和c-jun癌基因的 表达，有利于防止右心肥大和功能性衰竭。 总之，本实验初步探讨了钙信号系统与AS的发生发展的关系，表明钙信号系统参与了肉鸡 AS的发生发展。其次，证实PCNA以及癌基因在AS肉鸡右心肥大和功能衰竭过程中起着重要作用。 关键词 肉鸡腹水综合征，钙信号，Ca2+-ATPase，维拉帕米，增殖细胞核抗原，癌基因 Pulmonary Pulmonary hypertension(PH)and right ventricular hypertrophy have been commonly considered key roles in the incidence of ascites in broilers．Studies human showed that calcium signal transduction is basis of primary pulmonary hypertension and cardiac function failure．In addition，both cardiac muscle cell proliferation and proto-oncogene also important factors in cardiac hypertrophy and failure．At present，we have found calcium signal transduction and proto-oncogene may participate the ascites syndrome in broilers．In order to discuss thoroughly the mechanism of calcium signal f transduction and right ventricular hypertrophy and failure in broilers，we researched follow aspects： 1．Right ventricular hypertrophy and failure is important step in the development of ascites syndrome．Cytoplasmic calcium concentration is major regulator of cardiac contractile function and various physiological processes in cardiac muscle cells．The purpose of this study was to inquire the effects of verapamil，L—type voltage-dependent Ca2+channel blocker,on the right ventricle of ascitic broilers．Compared with birds under low temperature，the broilers given verapamil showed that the incidence of ascites decreased significantly,the AHI significantly decreased 29，36，43 and 50 days， the right ventricular systolic and diastolic pressure also significantly decreased 36 and 43 days，and the R