促甲状腺激素释放激素结合高压氧对大鼠神经病理性痛治疗作用的研究麻醉学专业论文.docxVIP

西南医科大学硕士学位论文阳性细胞核数量下降最多；大鼠脊髓背角浅层星形胶质细胞能够被坐 西南医科大学硕士学位论文 阳性细胞核数量下降最多；大鼠脊髓背角浅层星形胶质细胞能够被坐 骨神经压榨激活，相较于CCI组，TRH、HBO和HBO+TRH治疗均 能有效降低CCI大鼠术后第14、28天的脊髓背角浅层的GFAP阳性 细胞核数量，但HBO+TRH治疗组抑制率最高。(3)western blot得 到的结果同免疫组化是相同的，相较于CCI组，TRH、HBO和 HBO+TRH治疗均能有效抑制CCI大鼠脊髓GFAP蛋白的表达，但 HBO+TRH治疗组抑制率最高。结论：(1)TRH结合HBO治疗神经 病理性痛最为有效，可长时程降低CCI大鼠热痛敏和机械性痛觉超 敏的严重程度，缩短病程。(2)TRH结合HBO治疗能更有效的抑制 CCI引起的c—Fos表达量增加，同时还能能够有效抑制术侧脊髓背角 星形胶质细胞活化，从分子生物标志物水平进一步验证了TRH结合 HBO治疗的镇痛作用。 关键词：神经病理性痛；高压氧；促甲状腺激素释放激素；神经损伤 万方数据 西南医科大学硕士学位论文EFFECT 西南医科大学硕士学位论文 EFFECT AND MECHAN-ISM STUDY oF THYROTROPIN—RELEASING HoRMoNE CoMBINED WITH HYPERBARIC oXYGEN TREATMENT FoR NEURoPATHIC PAIN ABSTRACT obj ective：The current studies were designed to investigate whether thyrotropin。releasing hormone(TRH)combined with hyperbaric oxygen (HBO)treatment could attenuate neuropathic pain in CCI rats and to reverse or interrupt the injury—induced processing of nociception．Methods： (1)5 0 rats were divided into sham operation group(S group)，CCI model group(CCI group)，thyrotropin releasing hormone therapy group fCCI+ TRH group)，hyperbaric oxygen therapy group(CCI+HBO)，and combined treatment group(CCI+HBO+Tin4)．Thermal hyperalgesia was assessed by measuring foot withdrawal latency to heat stimulation． Mechanical allodynia was determined by measuring incidence of foot withdrawal in response to mechanical indentation of the plantar surface of each hind paw with Von Frey filaments．(2)Immunofluorescence staining was used to observe the state of c—fos expression and activation of astrocytes in superficial dorsal horn．The Western Blot method was used to detect GFAP protein expression levels in rat spinal cord．Result：(1)TRH， HBO and HBO+TRH CCI treatment can effectively relieve neuropathic 3 万方数据 西南医科大学硕士学位论文pain 西南医科大学硕士学位论文 pain in rats，but HBO+TRH treatment effect was more significant and significantly reduced severity and shortened duration of thermal hyperalgesia and mechanical allodynia．(2)Compared to the S group，the c-Fos expression of superficial