气相色谱-质谱法测定莲子中农药残留一测多评研究.PDFVIP

国际中医中药杂志2018年10月第40卷第10期 Int J Trad Chin Med, October 2018, Vol. 40, No.10 ·959 · ·实验与方药 · 气相色谱-质谱法测定莲子中 农药残留的一测多评研究 唐志康 余敏灵 朱敏凤 张富东 【摘要】 目的 建立气相色谱-质谱(gas chromatography-mass spectrometer, GC-MS/MS)测定莲子中 联苯菊酯等 3 种拟除虫菊酯类农药残留的一测多评方法。方法 采用乙腈提取样品，经 Cleanert TPH 专 用净化柱净化。采用 GC-MS/MS 测定样品，根据色谱相对保留时间和质荷比信息对样品进行定性分析， 以联苯菊酯二级离子扫描峰(181.1/166.1)面积为参照峰，计算甲氰菊酯二级离子扫描峰(265.1/210.1)面 积、溴氰菊酯二级离子扫描峰(252.9/93.0)面积与其面积之间的相对校正因子，建立以联苯菊酯为对照品， 测定莲子中联苯菊酯、甲氰菊酯、溴氰菊酯 3 种农药残留量的 GC-MS/MS 方法。结果 联苯菊酯、甲氰 菊酯、溴氰菊酯进样量在 0.01～0.1 ng 范围内与其色谱峰面积均有较好的线性关系，最低检出限(以进样 -4 -4 -3 量计)联苯菊酯为 4.321 ×10 ng ，甲氰菊酯为 3.435 ×10 ng ，溴氰菊酯为 8.913 ×10 ng 。平均回收率联 苯菊酯为 93.5%，甲氰菊酯为 93.5%，溴氰菊酯为 93.8% 。结论 该方法简便、准确、快速，适用于分 析莲子中联苯菊酯、甲氰菊酯、溴氰菊酯 3 种农药残留情况。 【关键词】 莲子；气相色谱-质谱；联苯菊酯；甲氰菊酯；溴氰菊酯；农药残留；一测多评 Study on the simultaneous measurement of residual level of three pesticides in nelumbinis semen using GC-MS/MS * * Tang Zhikang , Yu Minling, Zhu Minfeng, Zhang Fudong. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China Corresponding author: Yu Minling, Sichuan Leshan for Food and Drug Control, Leshan 614000, China; Email: 【Abstract 】 Objective To establish a quantitative method for the simultaneous measurement of the residual level of three pesticides in Nelumbinis semen by GC-MS/MS. Methods The samples were extracted by acetonitrile and purify by Cleanert TPH column. The samples were then tested by GC-MS/MS. Information on relative retention time and mass charge ratio was