磁场对酶及酶促反应过程的影响化学工程专业论文.docxVIP

摘要为了探究磁场对酶的影响，本文选取过氧化氢酶、胰蛋白酶、a一淀粉酶和脲 摘要 为了探究磁场对酶的影响，本文选取过氧化氢酶、胰蛋白酶、a一淀粉酶和脲 酶为研究对象，在不同磁场强度的永磁场中，对目标酶系进行一定时间的磁化处 理，然后从酶的活性、构象、酶促反应动力学等几方面进行考察。研究结果表 明，Jl-鸯l：l磁场对不溺的酶会产生不同的影响，对于过氧化氢酶，磁化后，酶的活 性明显增加，酶促反应的速度增加，巍交大，同时‰变大，二级结构也发生了 变化；对于胰蛋白酶磁场效应不是很明显，对活性、酶促反应动力学基本没有影 响，但是二级结构发生了一些变化；泓淀粉酶经磁化后，酶的活性也增大，但是 和过氧化氢酶不同的是，‰减小，‰减小，同时酶的二级结构也发生了一定的 变化：对于脲酶，磁化后，酶的活性也略有所增加，‰减小，‰减小，餐是没 有鼢淀粉酶明显。此外，磁场对某个酶的影响并不是呈简单的线性关系，邸随羞 磁场强度的增大，对酶|》g影响并不是线性增加。 本文分别对不同磁场强度下酶促反应动力学的实验数据进行了拟合，得到各 个酶的米氏常数，并对拟合结果进行了检验。 本文针对所研究的物系，从磁场对电荷的作用及分子在磁场中的受力分析入 手，讨论了磁场对酶的绐构的影响，并对磁场影响酶的机理进行了探讨。认为磁 场主要是通过影响酶的结构，从丽影响酶促反应，并以此为依据，对实验结果 进行了分析。 关键词：磁场；过氧化氢酶；胰蛋白酶；静淀粉酶；脲酶；酶促反应；酶的构象 ABSTRACTIn ABSTRACT In the study,in order to fmd the influence of the magnetic field On enzyme， catalase，trypsin，0【一amylase and urease were discussed under the various intensity static magnetic field which were 0。1 5T、0．30T and 0．45T with the control。强o enzyme was put in the magnetic field for some time and then the enzyme conformation．activity and enzymatic reactions were investigated．The results showed that the magnetic field would have different effects on the different enzyme，For the catalase，the activity Was obviously improved by the magnetic field，the velocity of enzymatic reaction WaS increased as well，the value of‰and‰were both enhanced． The enzyme conformation especially secondary structure which Was detected by the CD Was changed after magnetization．For the trypsin，this sort of magnetic effect was not very obvious，basically,there was no difference between the magnetic and the control in enzyme activity and enzymatic reactions，however,the secondary structure of the conformation Was changed by some extent．On the contrary,compared with the catalase，the enzymatic reactions value‰and‰of the a—amylase was decreased after magnetization，while the enzyme activi哆was still much higher than the control， the enzyme secondary structure’S change Was also observed．For the urease，the influence of the magnetic field Was similar to the a-amylase，however this e