《SNT1200-2003-烟草中转基因成分定性PCR检测方法》.pdfVIP

Protocol of qualitative PCR for the detection of genetically modified components in tobacco a3=. 3.2. 13 TrisI tris (hydroxymethyl) arninomethane,Z(% 4 S)JfilSEflti2 a 3.2. 14 DNA I de~x~ribonucleicacid,li##@#$I@. 3.2. 15 dNTPs 1 dTTP,dATP,dCTP,dGTP. 3.2. 16 dTTP I deoxyribonucleoside triphosphate,M#@VZ#l@ 3.2. 17 dATPl deoxyadenosine triphosphate,ll#@BP3@Ra 3.2. 18 dCTPl deoxycytidlne triphosphate,W#lekB3fEl#l@. 3.2. 19 dGTPl deoxyguanosine k p h o s p h a t e , % % 4 ~ ~ @ @ 3.2.20 dUTPIdeoxyuridine triphoaphate,lR#R%Z119B!. 3.2.21 bptbase pair,Xlf. fQaH#sAt7SH###tlP#$d@W7sb.k9rcHdBflBA ,#BJ $$8.$! N@###aH*?@@#BekQ, #.@#, BR*fl,P;f *I%#. RH3244fi RMB#ESHaHRSB@BMNSHB@HH@@nBaF*k?,B#BeSMSfi B~##ESMbC#LiSH;J$BBUWa NF#SBM5619S8H83iM@J3=91%i35sG?,NOs @ k P N B#SESB; NPT-I1r # !!,i$l bb krtfal$ IEI S iA TMV-CP, CMV-CP, TMV-54KD, RT, PVY-CP. 6 . 1 *B?@miH$M8GB/T 6682 43~~7JC(Z%*)M)5#5. 6 . 2 - F n ~ B l ~ * ~ j i f Q ~ # ~ ~ ~ ~ ~ ~ ~ N ~ 6,2.1 akB,Z~~B.#r#gt,CTAB,%%,EDTA,Tris,BQ@,P%~iFiLe 6 . 2 . 2 dNTPa*TaqDNA fltf3sBC,bl~#f~t#~ZBi!,DNAj).?.it6lE.%4#@?4%. 6 . 3 X#ZPE@Ylllkn(%%1). 1 BR90SBatarllA,*ZRS~IJiR3I#Afla ~ 1 2 6 R 851?RH 91 ¶b @ PJ Ftll#.tf;B bB % 5-gctcctacaaatgccatca-3 35s 9C n 19%~ Blk39t;llW 5-grtagtgggattgtgcgtca-3 5-gaatcctgttgccggtcttg3 NOS )hi# . l8obp